从普利亚地区采集的样本中 spp. 的检测。
Detection of spp. in Samples Collected from Apulia Region.
作者信息
Bonerba Elisabetta, Mottola Anna, Parisi Antonio, Di Pinto Angela, Serraino Andrea, Bozzo Giancarlo, Giacometti Federica, Ceci Edmondo, Tantillo Giuseppina
机构信息
Dipartimento di Medicina Veterinaria, Università di Bari , Valenzano (BA).
Istituto Zooprofilattico Sperimentale della Puglia e Basilicata , Putignano (BA).
出版信息
Ital J Food Saf. 2015 Feb 17;4(1):4583. doi: 10.4081/ijfs.2015.4583. eCollection 2015 Feb 3.
The aim of the study was to evaluate the occurrence of spp. in 20 samples of purchased at fish markets in Apulia region. The detection of spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mCCD) agar supplemented with Cefoperazone, Amphotericin B and Teicoplanin (CAT). In 6 out of the 20 tested samples the presence of spp. was found and confirmed by genus-based polymerase chain reaction. All the isolates were identified as belonging to the species using 16S rDNA sequencing and BLAST online. The results represent the first report in Italy of detection in marketed . The survey underlines the epidemiological importance of as an emerging pathogen, and highlights that mussels should be considered as a potential cause of foodborne disease outbreak.
本研究的目的是评估在普利亚地区鱼市购买的20份[具体名称未给出]样本中[具体物种未给出]属物种的出现情况。在添加了头孢哌酮、两性霉素B和替考拉宁（CAT）的改良木炭头孢哌酮脱氧胆酸盐（mCCD）琼脂上进行选择性富集后，对[具体物种未给出]属物种进行检测。在20个测试样本中的6个样本中发现了[具体物种未给出]属物种的存在，并通过基于属的聚合酶链反应得到证实。使用16S rDNA测序和在线BLAST将所有分离株鉴定为属于[具体物种名称未给出]物种。这些结果代表了意大利首次关于在市售[具体名称未给出]中检测到[具体物种未给出]的报告。该调查强调了[具体物种未给出]作为一种新兴病原体的流行病学重要性，并强调贻贝应被视为食源性疾病暴发的潜在原因。
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