五种分子方法监测弯曲菌属的性能研究
Performance of five molecular methods for monitoring Arcobacter spp.
机构信息
Unitat de Microbiologia, Departament de Ciències Mediques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
出版信息
BMC Microbiol. 2013 Oct 3;13:220. doi: 10.1186/1471-2180-13-220.
BACKGROUND
Bacteria belonging to the Arcobacter genus are emerging enteropathogens and potential zoonotic agents. Their taxonomy has evolved very rapidly, and there are presently 18 recorded species. The prevalence of species belonging to Arcobacter is underestimated because of the limitations of currently available methods for species identification.The aim of this study was to compare the performance of five PCR based methods that target regions of 16S rRNA, 23S rRNA or gyrA genes to identify Arcobacter species, and to review previous results reported in the literature using these methods.
RESULTS
The five tested methods were found not to be reliable. They misidentified between 16.8% and 67.4% of the studied strains; this was dependent upon the target regions of the tested genes. The worst results obtained were for the identification of Arcobacter cryaerophilus and Arcobacter butzleri when the 23S rRNA gene was used as the target. These species were confused with many non-targeted species.
CONCLUSION
Our results suggest that the known diversity of Arcobacter spp. in different environments could be expanded if reliable identification methods are applied in future studies.
背景
弧菌属细菌是新兴的肠道病原体和潜在的人畜共患病原体。它们的分类学发展非常迅速，目前已有 18 个记录物种。由于目前用于物种鉴定的方法存在局限性，因此弧菌属物种的流行情况被低估了。本研究旨在比较五种基于 PCR 的方法在鉴定弧菌属物种方面的性能，这些方法针对 16S rRNA、23S rRNA 或 gyrA 基因的区域，并回顾使用这些方法在文献中报道的先前结果。
结果
五种测试方法被发现不可靠。它们错误鉴定了 16.8%至 67.4%的研究菌株；这取决于测试基因的目标区域。使用 23S rRNA 基因作为目标时，获得的最差结果是对嗜冷弧菌和 Butzleri 弧菌的鉴定。这些物种与许多非目标物种混淆。
结论
我们的结果表明，如果在未来的研究中应用可靠的鉴定方法，不同环境中弧菌属的已知多样性可能会扩大。
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