Birmpa Angeliki, Kalogeropoulos Konstantinos, Kokkinos Petros, Vantarakis Apostolos
Environmental Microbiology Unit, Department of Public Health, University of Patras , Greece.
Ital J Food Saf. 2015 Aug 28;4(3):5383. doi: 10.4081/ijfs.2015.5383. eCollection 2015 Jun 30.
In the present study, the effectiveness of two loop-mediated isothermal amplification (LAMP) assays was evaluated. Samples of romaine lettuce, strawberries, cherry tomatoes, green onions and sour berries were inoculated with known dilutions (10-10 CFU/g of produce) of Enteritidis and With LAMP, assay pathogens can be detected in less than 60 min. The limits of detection of Enteritidis and depended on the food sample tested and on the presence of enrichment step. After enrichment steps, all food samples were found positive even at low initial pathogen levels. The developed LAMP, assays, are expected to become a valuable, robust, innovative, powerful, cheap and fast monitoring tool, which can be extensively used for routine analysis, and screening of contaminated foods by the food industry and the Public Food Health Authorities.
在本研究中，评估了两种环介导等温扩增（LAMP）检测方法的有效性。用已知稀释度（每克农产品10 - 10 CFU）的肠炎沙门氏菌接种生菜、草莓、樱桃番茄、青葱和酸浆果样本。使用LAMP检测方法，可在不到60分钟内检测到病原体。肠炎沙门氏菌的检测限取决于所测试的食品样本以及是否存在富集步骤。经过富集步骤后，即使初始病原体水平较低，所有食品样本均呈阳性。所开发的LAMP检测方法有望成为一种有价值、稳健、创新、强大、廉价且快速的监测工具，可广泛用于食品行业和公共食品卫生当局的常规分析以及受污染食品的筛查。
