Zhuang Linlin, Gong Jiansen, Zhang Ping, Zhang Di, Zhao Ying, Yang Jianbo, Liu Guofang, Zhang Yu, Shen Qiuping
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, People's Republic of China.
State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering and Basic Medicine Research and Innovation Center of Ministry of Education, Zhongda Hospital, Southeast University, Nanjing, 211102, People's Republic of China.
Discov Nano. 2024 Aug 6;19(1):124. doi: 10.1186/s11671-024-04075-9.
Salmonella, the prevailing zoonotic pathogen within the Enterobacteriaceae family, holds the foremost position in global bacterial poisoning incidents, thereby signifying its paramount importance in public health. Consequently, the imperative for expeditious and uncomplicated detection techniques for Salmonella in food is underscored. After more than two decades of development, loop-mediated isothermal amplification (LAMP) has emerged as a potent adjunct to the polymerase chain reaction, demonstrating significant advantages in the realm of isothermal amplification. Its growing prominence is evident in the increasing number of reports on its application in the rapid detection of Salmonella. This paper provides a systematic exposition of the technical principles and characteristics of LAMP, along with an overview of the research progress made in the rapid detection of Salmonella using LAMP and its derivatives. Additionally, the target genes reported in various levels, including Salmonella genus, species, serogroup, and serotype, are summarized, aiming to offer a valuable reference for the advancement of LAMP application in Salmonella detection. Finally, we look forward to the development direction of LAMP and expect more competitive methods to provide strong support for food safety applications.
沙门氏菌是肠杆菌科中主要的人畜共患病原体，在全球细菌性中毒事件中位居首位，这表明其在公共卫生方面至关重要。因此，强调了快速、简便的食品中沙门氏菌检测技术的必要性。经过二十多年的发展，环介导等温扩增技术（LAMP）已成为聚合酶链反应的有力辅助技术，在等温扩增领域显示出显著优势。其在沙门氏菌快速检测中的应用报道日益增多，凸显了其日益重要的地位。本文系统阐述了LAMP的技术原理和特点，概述了利用LAMP及其衍生技术快速检测沙门氏菌的研究进展。此外，总结了不同层面（包括沙门氏菌属、种、血清群和血清型）报道的靶基因，旨在为LAMP在沙门氏菌检测中的应用发展提供有价值的参考。最后，展望了LAMP的发展方向，期待有更多具有竞争力的方法为食品安全应用提供有力支持。
