Pacak Andrzej, Kruszka Katarzyna, Swida-Barteczka Aleksandra, Nuc Przemyslaw, Karlowski Wojciech, Jarmolowski Artur, Szweykowska-Kulinska Zofia
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
Department of Computational Biology, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznań, Poland.
Acta Biochim Pol. 2016;63(4):799-809. doi: 10.18388/abp.2016_1347. Epub 2016 Nov 2.
MicroRNAs are 19- to 24-nt-long single-stranded RNAs that are crucial regulators of gene expression which control plant development and response to environmental cues. We have analyzed microtranscriptomes of five barley developmental stages. Generally, during the barley development, miR168-3p and miR1432-5p levels increase while the 5'U-miR156-5p level decreases (with exception for the 2-week-old barley). We have identified two miR156-5p izomiRs (called 5'U-miR156-5p [20 nt] and 5'UU-miR156-5p [21 nt]), which were expressed differently during barley development. The 5' U-miR156-5p level decreased in 3-week-, 6-week-, and 68-day-old barley, when compared to the 1-week-old plants. Meanwhile, the 5' UU-miR156-5p level increased significantly in the 68-day-old barley plants. Moreover, only the 5' U-miR156 isomiR recognizes and guides unique transcription factor mRNAs from the Squamosa Promoter Binding Protein-Like (SPL) family. We identified many non-canonical microRNAs with changed expression levels during the barley development. Here, we present the profiles of microRNA expression characteristics for particular barley developmental stages. These analyses are accompanied by the experimental degradome analysis of miRNA targets.
微小RNA是长度为19至24个核苷酸的单链RNA，是基因表达的关键调节因子，控制着植物的发育以及对环境信号的响应。我们分析了大麦五个发育阶段的微小转录组。一般来说，在大麦发育过程中，miR168 - 3p和miR1432 - 5p的水平升高，而5'U - miR156 - 5p的水平降低（两周大的大麦除外）。我们鉴定出两种miR156 - 5p的异源异构体（称为5'U - miR156 - 5p [20个核苷酸]和5'UU - miR156 - 5p [21个核苷酸]），它们在大麦发育过程中的表达有所不同。与一周大的植株相比，5'U - miR156 - 5p在三周、六周和68天大的大麦中的水平降低。同时，5'UU - miR156 - 5p在68天大的大麦植株中的水平显著升高。此外，只有5'U - miR156异源异构体能够识别并引导来自Squamosa启动子结合蛋白样（SPL）家族的独特转录因子mRNA。我们鉴定出许多在大麦发育过程中表达水平发生变化的非经典微小RNA。在这里，我们展示了特定大麦发育阶段微小RNA表达特征的概况。这些分析伴随着对miRNA靶标的实验性降解组分析。
