a Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology , Adam Mickiewicz University , Poznań , Poland.
b Institute for Integrative Biology of the Cell, Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique , Université Paris-Sud, Université Paris-Saclay , Gif-sur-Yvette , France.
RNA Biol. 2018;15(7):886-891. doi: 10.1080/15476286.2018.1481695. Epub 2018 Jul 14.
In this article a novel mechanism of retrograde signaling by chloroplasts during stress is described. This mechanism involves the DNA/RNA binding protein WHIRLY1 as a regulator of microRNA levels. By virtue of its dual localization in chloroplasts and the nucleus of the same cell, WHIRLY1 was proposed as an excellent candidate coordinator of chloroplast function and nuclear gene expression. Comparison of wild-type and transgenic plants with an RNAi-mediated knockdown of WHIRLY1 showed, that the transgenic plants were unable to cope with continuous high light conditions. They were impaired in production of several microRNAs mediating post-transcriptional responses during stress. The results support a central role of WHIRLY1 in retrograde signaling and also underpin a so far underestimated role of microRNAs in this process.
本文描述了叶绿体在胁迫下逆向信号传递的一种新机制。该机制涉及 DNA/RNA 结合蛋白 WHIRLY1 作为 microRNA 水平的调节剂。由于其在叶绿体和同一细胞的细胞核中的双重定位，WHIRLY1 被提议作为叶绿体功能和核基因表达的优秀协调者。对具有 RNAi 介导的 WHIRLY1 敲低的野生型和转基因植物的比较表明，转基因植物无法应对连续的高光条件。它们在逆境中产生几种介导转录后反应的 microRNAs 的能力受损。结果支持 WHIRLY1 在逆行信号传递中的核心作用，并也支持 microRNAs 在该过程中的作用被低估。
