Harata Gaku, He Fang, Takahashi Kyoko, Hosono Akira, Miyazawa Kenji, Yoda Kazutoyo, Hiramatsu Masaru, Kaminogawa Shuichi
Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama 241-0023, Japan.
Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan.
Microorganisms. 2016 Oct 27;4(4):40. doi: 10.3390/microorganisms4040040.
Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinal with mast cells is still necessary. Twenty-three strains of , including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the tested characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and GG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest that , particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism.
肥大细胞在免疫球蛋白E（IgE）介导的过敏性疾病中起关键作用，肥大细胞的脱颗粒在这些疾病的发病机制中很重要。肠道微生物群的紊乱，尤其是内源性乳酸菌的紊乱，可能是IgE介导的过敏性疾病的一个促成因素。关于人类肠道与肥大细胞相互作用的更多知识仍然是必要的。基于β-己糖胺酶释放试验，测试了23株细菌，包括商业菌株、参考菌株和来自人类肠道的菌株，它们在体外调节大鼠嗜碱性白血病RBL-2H3细胞（RBL-2H3）脱颗粒的能力。每种测试菌株都能特异性抑制RBL-2H3细胞的IgE介导的脱颗粒，其中GG菌株对细胞的抑制作用最强。此外，与参考菌株相比，从人类肠道分离的细菌显著抑制了RBL-2H3细胞的脱颗粒。这些结果表明，细菌，尤其是来自人类肠道的细菌，可以以菌株依赖的方式影响肥大细胞的激活。应该进行进一步的研究来分析其作用机制。
