核糖核酸酶抑制剂基因敲除使人类细胞易受分泌型核糖核酸酶的影响。
Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases.
作者信息
Thomas Sydney P, Kim Eunji, Kim Jin-Soo, Raines Ronald T
机构信息
Graduate Program in Cellular & Molecular Biology, University of Wisconsin-Madison , 1525 Linden Drive, Madison, Wisconsin 53706, United States.
Center for Genome Engineering, Institute for Basic Science , Seoul 08826, Republic of Korea.
出版信息
Biochemistry. 2016 Nov 22;55(46):6359-6362. doi: 10.1021/acs.biochem.6b01003. Epub 2016 Nov 8.
Abstract
Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases.
摘要
核糖核酸酶抑制剂（RNH1）是一种胞质蛋白，它以飞摩尔亲和力与人核糖核酸酶1（RNase 1）及同源分泌型核糖核酸酶结合。RNH1含有32个半胱氨酸残基，并被认为具有抗氧化作用。在此，我们使用CRISPR-Cas9敲除HeLa细胞中的RNH1。我们发现，细胞内的RNH1能显著保护细胞免受RNase 1致死性核糖核酸酶活性的影响，但不能保护细胞免受氧化剂的影响。我们得出结论，RNH1可保护胞质RNA免受入侵核糖核酸酶的破坏。
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