Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hong Kong, China.
Sci Rep. 2016 Nov 3;6:36327. doi: 10.1038/srep36327.
Recently zwitterionic materials have been widely applied in the biomedical and bioengineering fields due to their excellent biocompatibility. Inspired by these, this study presents a graft-to strategy via covalent bond formation to fabricate safe and durable antibacterial textile surfaces. A novel zwitterionic sulfobetaine containing triazine reactive group was specifically designed and synthesized. MTT assay showed that it had no obvious cytotoxicity to human skin HaCaT cells as verified by ca. 89.9% relative viability at a rather high concentration of 0.8 mg·mL. In the evaluation for its skin sensitization, the maximum score for symptoms of erythema and edema in all tests were 0 in all observation periods. The sulfobetaine had a hydrophilic nature and the hydrophilicity of the textiles was enhanced by 43.9% when it was covalently grafted onto the textiles. Moreover, the textiles grafted with the reactive sulfobetaine exhibited durable antibacterial activities, which was verified by the fact that they showed antibacterial rates of 97.4% against gram-positive S. aureus and 93.2% against gram-negative E. coli even after they were laundered for 30 times. Therefore, the titled zwitterionic sulfobetaine is safe to human for healthcare and wound dressing and shows a promising prospect on antibacterial textile application.
近年来，由于其优异的生物相容性，两性离子材料在生物医学和生物工程领域得到了广泛的应用。受此启发，本研究通过共价键形成提出了一种接枝策略，以制造安全且耐用的抗菌纺织品表面。专门设计和合成了一种含有三嗪反应基团的新型两性离子磺酸甜菜碱。MTT 试验表明，它对人皮肤 HaCaT 细胞没有明显的细胞毒性，在相当高的浓度 0.8 mg·mL 时，相对活力约为 89.9%。在其皮肤致敏性评价中，所有测试中红斑和水肿症状的最大评分在所有观察期内均为 0。磺酸甜菜碱具有亲水性，当它共价接枝到纺织品上时，纺织品的亲水性提高了 43.9%。此外，接枝反应性磺酸甜菜碱的纺织品表现出持久的抗菌活性，即使经过 30 次洗涤后，它们对革兰氏阳性菌金黄色葡萄球菌的抗菌率仍为 97.4%，对革兰氏阴性菌大肠杆菌的抗菌率仍为 93.2%，这一事实证明了这一点。因此，标题中的两性离子磺酸甜菜碱对人体用于医疗保健和伤口敷料是安全的，并在抗菌纺织品应用方面显示出有希望的前景。
