Chou Ying-Nien, Wen Ten-Chin, Chang Yung
Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Acta Biomater. 2016 Aug;40:78-91. doi: 10.1016/j.actbio.2016.03.046. Epub 2016 Apr 1.
Most biomaterials have a lack of a simple, efficient and robust antifouling modification approach that limits their potential for biomedical applications. The challenge is to develop a universal surface grafting solution to meet the antifouling requirement. In this work, a new formulation of zwitterionic sulfobetaine-based copolymer, ploy(glycidyl methacrylate-co-sulfobetaine methacrylate) (poly(GMA-co-SBMA)), is designed as a chemical for grafting onto material and is introduced for the surface zwitterionization of versatile biomaterials, including ceramic, metal, and plastics. The grafting principle used to stabilize the poly(GMA-co-SBMA) on the target surfaces is based the base-induced ring opening reaction between epoxied and hydroxyl groups. A universal surface modification procedure was developed and performed from an optimized sequence of ultra-violet ozone pretreatment and trimethylamine-catalyzed zwitterionization on a selective case of versatile surfaces including silicon wafer, ceramic glass, titanium, steel, and polystyrene. The prepared poly(GMA-co-SBMA) with an optimum PGMA/PSBMA ratio of 0.23 and a molecular weight of 25kDa exhibited the best resistance to fibrinogen adsorption with over 90% reduction as well as blood cell activation, tissue cell adhesion and bacterial attachment on the zwitterionic copolymer grafted surfaces. The developed antifouling grafting introduces a universal modification method to generate zwitterionic interfaces on versatile biomaterial substrates, providing great potential for application in medical device coating.
A simple, efficient and robust antifouling modification approach is critical for many scientific interests and industrial applications. In current stage, the existing available zwitterionic modifications suffer from the lack of universal surface grafting solution to achieve the antifouling requirement on versatile biomaterial substrates. In this study, we synthesized and characterized a new zwitterionic sulfobetaine-based copolymer, ploy(glycidyl methacrylate-co-sulfobetaine methacrylate) (poly(GMA-co-SBMA)), which is designed as chemical grafting onto material and introduced for the surface zwitterionization of versatile biomaterials, including ceramic, metal, and plastics. This research have a promising opportunity for the application of stealth biomaterial interfaces on the next generation of medical devices.
大多数生物材料缺乏一种简单、高效且强大的防污改性方法，这限制了它们在生物医学应用中的潜力。挑战在于开发一种通用的表面接枝解决方案以满足防污要求。在这项工作中，一种基于两性离子磺基甜菜碱的新型共聚物配方，聚（甲基丙烯酸缩水甘油酯 - 甲基丙烯酸磺基甜菜碱）（聚（GMA - co - SBMA）），被设计为一种用于接枝到材料上的化学品，并被引入用于包括陶瓷、金属和塑料在内的多种生物材料的表面两性离子化。用于在目标表面上稳定聚（GMA - co - SBMA）的接枝原理基于环氧基和羟基之间的碱诱导开环反应。通过对包括硅片、陶瓷玻璃、钛、钢和聚苯乙烯在内的多种表面进行优化的紫外线臭氧预处理和三甲胺催化的两性离子化序列，开发并实施了一种通用的表面改性程序。制备的聚（GMA - co - SBMA），其最佳PGMA/PSBMA比例为0.23，分子量为25kDa，在两性离子共聚物接枝表面上对纤维蛋白原吸附的抗性最佳，减少超过90%，以及对血细胞活化、组织细胞粘附和细菌附着也有很好的抗性。所开发的防污接枝引入了一种通用的改性方法，以在多种生物材料基材上生成两性离子界面，为医疗设备涂层应用提供了巨大潜力。
一种简单、高效且强大的防污改性方法对于许多科学兴趣和工业应用至关重要。在现阶段，现有的两性离子改性缺乏通用的表面接枝解决方案，无法在多种生物材料基材上实现防污要求。在本研究中，我们合成并表征了一种新型的基于两性离子磺基甜菜碱的共聚物，聚（甲基丙烯酸缩水甘油酯 - 甲基丙烯酸磺基甜菜碱）（聚（GMA - co - SBMA）），它被设计为接枝到材料上的化学品，并被引入用于包括陶瓷、金属和塑料在内的多种生物材料的表面两性离子化。这项研究为下一代医疗设备上隐形生物材料界面的应用提供了一个有前景的机会。
