Xu H M, Cui Y Z, Wang W G, Cheng H X, Sun Y J, Zhao H Y, Yan Y Q
Department of Emergency Medicine, Children's Hospital of Kaifeng City, Kaifeng, China
Department of Hemopathology, Children's Hospital of Kaifeng City, Kaifeng, China.
Genet Mol Res. 2016 Oct 5;15(4):gmr8705. doi: 10.4238/gmr.15048705.
The aim of this study was to investigate the expression and clinical significance of the obesity-associated gene STEAP4 in obese children. Fifty-three obese children and 33 children with a standard body weight (control) from our hospital were recruited to this study. The expression of STEAP4 mRNA and protein in the adipose tissue were detected by reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay, respectively, in order to analyze the relationship between STEAP4 mRNA and protein levels and blood pressure, blood lipid profile, blood glucose levels, and inflammation in obese children. Obese children showed significantly lower levels of STEAP4 mRNA and protein in the adipose tissue compared to the control subjects (P < 0.05). The obese subjects exhibited significantly higher diastolic blood pressure (DBP), systolic blood pressure (SBP), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), fasting plasma glucose (FPG), interleukin (IL)-6, and tumor necrosis factor (TNF)-α levels, and a significantly lower high-density lipoprotein (HDL) level, compared to the control subjects (P < 0.05). Correlation analysis revealed that STEAP4 expression was negatively correlated with the DBP, SBP, TC, TG, LDL, FPG, IL-6, and TNF-α levels, and was positively correlated with the HDL level (P < 0.05). In conclusion, the expression of STEAP4 was significantly downregulated in the adipose tissue of obese children and was closely related to the blood pressure, blood lipid, blood glucose, and inflammation in these patients; therefore, these results could provide a theoretical basis for the treatment of childhood obesity.
本研究旨在探讨肥胖相关基因STEAP4在肥胖儿童中的表达及其临床意义。本研究招募了我院53名肥胖儿童和33名体重标准的儿童（对照组）。分别采用逆转录聚合酶链反应和酶联免疫吸附测定法检测脂肪组织中STEAP4 mRNA和蛋白的表达，以分析肥胖儿童STEAP4 mRNA和蛋白水平与血压、血脂谱、血糖水平及炎症之间的关系。与对照组相比，肥胖儿童脂肪组织中STEAP4 mRNA和蛋白水平显著降低（P<0.05）。与对照组相比，肥胖受试者的舒张压（DBP）、收缩压（SBP）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、空腹血糖（FPG）、白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α水平显著升高，高密度脂蛋白（HDL）水平显著降低（P<0.05）。相关性分析显示，STEAP4表达与DBP、SBP、TC、TG、LDL、FPG、IL-6和TNF-α水平呈负相关，与HDL水平呈正相关（P<0.05）。总之，肥胖儿童脂肪组织中STEAP4表达显著下调，且与这些患者的血压、血脂、血糖及炎症密切相关；因此，这些结果可为儿童肥胖症的治疗提供理论依据。
