Eralp Ayhan, Menguc Nur Yumusak, Polat Elif, Yuncu Mehmet, Koruk Mehmet, Demir Serap Sergul Inaloz, Sari İbrahim
Histology and Embryology, Medical Faculty, Ataturk University, Erzurum,. Turkey.
Endocr Metab Immune Disord Drug Targets. 2016;16(3):205-212. doi: 10.2174/1871530316666161029205427.
Preventing liver damage that might lead to cirrhosis is very important in the early stages of injury to that organ. The role of mast cells (MCs) in liver injuries has been long debated, and vitamin E is a well-known antioxidant used to treat those injuries. This study aimed to determine the protective role of vitamin E on MCs in injury to the liver that is triggered by carbon tetrachloride (CCl4). There is a correlation between MC deposits and improvement in fibrosis tissues.
To further examine this, 68 male Albino Wistar rats were divided randomly into five groups: the control group, the vitamin E group, the CCl4 group, the CCl4 + vitamin E group, and the vitamin E + CCl4 group. Malondialdehyde (MDA) analysis, MC counts, histopathological investigation, and statistical analyses were used to evaluate the findings.
The administration of CCl4 resulted in an increase in the accumulation of MCs, degenerative parenchyma cells, MDA level, steatosis and inflammation. Additionally, proliferation of the bile ducts in the portal area and porto-portal and porto-central fibrosis were observed in the CCl4 group. In contrast, in the vitamin E group and in the groups administered a combination of vitamin E and CCl4, vitamin E prevented these increases.
It was concluded that the significant decrease in the MC counts, in the level of MDA and the number of degenerative cells, as well as a decrease in the steatosis and inflammation scores showed that vitamin E could prevent liver injuries by protecting the organ's histological architecture. Finally, the results indicate that vitamin E has positive effects on liver injuries.
在肝脏损伤的早期阶段，预防可能导致肝硬化的肝损伤非常重要。肥大细胞（MCs）在肝损伤中的作用长期以来一直存在争议，而维生素E是一种用于治疗这些损伤的著名抗氧化剂。本研究旨在确定维生素E对四氯化碳（CCl4）引发的肝损伤中MCs的保护作用。MCs沉积与纤维化组织的改善之间存在相关性。
为进一步研究此问题，将68只雄性白化Wistar大鼠随机分为五组：对照组、维生素E组、CCl4组、CCl4 + 维生素E组和维生素E + CCl4组。采用丙二醛（MDA）分析、MC计数、组织病理学研究和统计分析来评估研究结果。
给予CCl4导致MCs积聚增加、实质细胞变性、MDA水平升高、脂肪变性和炎症。此外，在CCl4组中观察到门管区胆管增生以及门管 - 门管和门管 - 中央纤维化。相比之下，在维生素E组以及给予维生素E和CCl4组合的组中，维生素E可防止这些增加。
得出的结论是，MC计数、MDA水平和变性细胞数量显著降低，以及脂肪变性和炎症评分降低，表明维生素E可通过保护肝脏的组织结构来预防肝损伤。最后，结果表明维生素E对肝损伤具有积极作用。
