El-Faras A A, Sadek I A, Ali Y E, Khalil Mim, Mussa E B
Department of Physiology, Medical Research Institute, Alexandria University , Alexandria, Egypt.
Department of Zoology, Faculty of Science, Alexandria University , Alexandria, Egypt.
Physiol Int. 2016 Jun 1;103(2):157-168. doi: 10.1556/036.103.2016.2.3.
The increased generation of free radicals plays an important role in testicular damage. The present study aimed to investigate the adverse effects of carbon tetrachloride (CCl) on the reproductive system of male rats as well as to examine whether Vitamin E (VE) is able to ameliorate these effects. The rats were equally divided into three groups: control, CCl-treated, and CCl + VE-treated groups. After 4 weeks of treatment, the decrease in body and testes weights, sperm parameters, and the decrease in serum levels of testosterone, luteinizing hormone, and follicle-stimulating hormone of CCl-treated rats were ameliorated by VE treatment. The co-administration of VE with CCl significantly decreased the level of lipid peroxidation production (malondialdehyde) and increased the activity of anti-oxidant enzymes (superoxide dismutase and catalase) when compared with the CCl group. Moreover, VE prevented CCl-induced severe testicular histopathological lesions and deformities in spermatogenesis. The results demonstrate that VE augments the anti-oxidants' defense mechanism against CCl-induced reproductive toxicity suggesting a therapeutic role in free radical-mediated infertility.
自由基生成增加在睾丸损伤中起重要作用。本研究旨在探讨四氯化碳（CCl）对雄性大鼠生殖系统的不良影响，以及研究维生素E（VE）是否能够改善这些影响。将大鼠平均分为三组：对照组、CCl处理组和CCl + VE处理组。治疗4周后，VE治疗改善了CCl处理大鼠的体重和睾丸重量下降、精子参数以及血清睾酮、黄体生成素和促卵泡激素水平的降低。与CCl组相比，VE与CCl共同给药显著降低了脂质过氧化产物（丙二醛）水平，并增加了抗氧化酶（超氧化物歧化酶和过氧化氢酶）的活性。此外，VE预防了CCl诱导的严重睾丸组织病理学损伤和精子发生畸形。结果表明，VE增强了抗氧化防御机制以对抗CCl诱导的生殖毒性，提示其在自由基介导的不育症中具有治疗作用。
