National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Beijing University of Technology, Beijing 100124, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Water Res. 2017 Jan 1;108:46-56. doi: 10.1016/j.watres.2016.10.051. Epub 2016 Oct 21.
In this study, a novel DEAMOX (DEnitrifying AMmonium OXidation) process coupling anammox with partial-denitrification generated nitrite (NO-N) from nitrate (NO-N) was developed for simultaneously treating ammonia (NH-N) and NO-N containing wastewaters. The performance was evaluated in sequencing batch reactors (SBRs) with different carbon sources for partial-denitrification: acetate (R1) and ethanol (R2). Long-term operation (180 days) suggested that desirable nitrogen removal was achieved in both reactors. The performance maintained stably in R1 despite the seasonal decrease of temperature (29.2 °C-12.7 °C), and high nitrogen removal efficiency (NRE) of 93.6% on average was obtained with influent NO-N to NH-N ratio (NO-N/NH-N) of 1.0. The anammox process contributed above 95% to total nitrogen (TN) removal in R1 with the nitrate-to-nitrite transformation ratio (NTR) of 95.8% in partial-denitrification. A little lower NRE was observed in R2 with temperature dropped from 90.0% at 22.7 °C to 85.2% at 16.6 °C due to the reduced NTR (87.0%-67.0%). High-throughput sequencing analysis revealed that Thauera genera were dominant in both SBRs (accounted for 61.53% in R1 and 45.17% in R2) and possibly played a key role for partial-denitrification with high NO-N accumulation. The Denitratisoma capable of complete denitrification (NO-N→N) was found in R2 that might lead to lower NTR. Furthermore, different anammox species was detected with Candidatus Brocadia and Candidatus Kuenenia in R1, and only Candidatus Kuenenia in R2.
在这项研究中，开发了一种新型的 DEAMOX（反硝化氨氧化）工艺，该工艺将厌氧氨氧化与部分反硝化相结合，从硝酸盐（NO-N）中生成亚硝酸盐（NO-N），用于同时处理含有氨（NH-N）和 NO-N 的废水。该工艺在不同碳源（乙酸盐（R1）和乙醇（R2））用于部分反硝化的序批式反应器（SBR）中进行了性能评估。长期运行（180 天）表明，两个反应器均实现了理想的脱氮效果。尽管温度（29.2°C-12.7°C）季节性下降，但 R1 的性能仍保持稳定，当进水 NO-N 与 NH-N 比（NO-N/NH-N）为 1.0 时，平均获得了 93.6%的高氮去除效率（NRE）。在 R1 中，厌氧氨氧化过程对总氮（TN）去除的贡献超过 95%，部分反硝化过程中的硝酸盐到亚硝酸盐的转化率（NTR）为 95.8%。由于 NTR（87.0%-67.0%）降低，当温度从 22.7°C 的 90.0%降至 16.6°C 的 85.2%时，R2 的 NRE 略有降低。高通量测序分析表明，Thauera 属在两个 SBR 中均占主导地位（R1 中占 61.53%，R2 中占 45.17%），并且可能在高 NO-N 积累的情况下对部分反硝化起关键作用。在 R2 中发现了具有完全反硝化能力（NO-N→N）的 Denitratisoma，这可能导致较低的 NTR。此外，在 R1 中检测到了不同的厌氧氨氧化种属，包括 Candidatus Brocadia 和 Candidatus Kuenenia，而在 R2 中仅检测到 Candidatus Kuenenia。
