Fan S G, Wei J F, Guo Y H, Huang G J, Yu D H
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Genet Mol Res. 2016 Nov 3;15(4):gmr-15-gmr15049054. doi: 10.4238/gmr15049054.
The pearl oyster Pinctada fucata is an important commercial marine shellfish that is cultured for producing saltwater pearls. In this study, 468 single nucleotide polymorphisms (SNPs) were screened from P. fucata transcriptome data, and 119 polymorphic SNPs were successfully isolated by a two-step small-amplicon high-resolution melting assay. Of these, 88 were annotated with BLAST in the Nr database and 90 were in the open reading frame, including 16 non-synonymous SNPs and 74 synonymous SNPs; 12 SNPs were in the 3'-untranslated region (UTR) and 1 was in the 5'-UTR. Twenty-five SNPs were randomly chosen to test the genetic diversity of 40 wild individuals from Liusha Bay, China. All of the loci had two alleles. The observed and expected heterozygosities ranged from 0.0417 to 0.6042 and from 0.2945 to 0.5053, respectively. Minor allele frequencies ranged from 0.1771 to 0.5000, and the polymorphism information content ranged from 0.2516 to 0.3750. These novel SNP markers can contribute to P. fucata genetics and breeding studies.
合浦珠母贝是一种重要的商业性海洋贝类，用于养殖海水珍珠。在本研究中，从合浦珠母贝转录组数据中筛选出468个单核苷酸多态性（SNP），并通过两步小扩增子高分辨率熔解分析成功分离出119个多态性SNP。其中，88个在Nr数据库中通过BLAST注释，90个位于开放阅读框中，包括16个非同义SNP和74个同义SNP；12个SNP位于3'非翻译区（UTR），1个位于5'UTR。随机选择25个SNP来检测来自中国流沙湾的40个野生个体的遗传多样性。所有位点均有两个等位基因。观察到的杂合度和预期杂合度分别为0.0417至0.6042和0.2945至0.5053。次要等位基因频率范围为0.1771至0.5000，多态性信息含量范围为0.2516至0.3750。这些新的SNP标记有助于合浦珠母贝的遗传学和育种研究。
