Antonov S A, Novosadova E V, Arsenyeva E L, Grefenstein M A, Zykova A A, Kobylyansky A G, Manuilova E S, Grivennikov I A, Illarioshkin S N, Myasoedov N F
Instiute of molecular genetics of Russian Academy of sciences, Moscow, Russia.
Scientific Center of Neurology, Moscow, Russia.
Dokl Biol Sci. 2016 Sep;470(1):244-246. doi: 10.1134/S0012496616050045. Epub 2016 Nov 8.
The influence of GABA receptor agonists on the terminal differentiation in vitro of dopaminergic (DA) neurons derived from IPS cells was investigated. GABA-A agonist muscimol induced transient elevation of intracellular Ca level ([Ca] ) in the investigated cells at days 5 to 21 of differentiation. Differentiation of cells in the presence of muscimol reduced tyrosine hydroxylase expression. Thus, the presence of active GABA-A receptors, associated with phenotype determination via Ca-signalling was demonstrated in differentiating human DA neurons.
研究了γ-氨基丁酸（GABA）受体激动剂对诱导多能干细胞（iPS细胞）来源的多巴胺能（DA）神经元体外终末分化的影响。在分化的第5至21天，GABA-A激动剂蝇蕈醇可诱导所研究细胞内的细胞内钙水平（[Ca] ）短暂升高。在蝇蕈醇存在的情况下，细胞分化会降低酪氨酸羟化酶的表达。因此，在人DA神经元分化过程中，证实了存在与通过钙信号传导决定表型相关的活性GABA-A受体。
