Johnson S R, Graham C H, Lysiak J J, Lala P K
Department of Anatomy, University of Western Ontario, London, Canada.
Am J Anat. 1989 May;185(1):9-18. doi: 10.1002/aja.1001850103.
The possible hemopoietic origin of certain precursors of uterine decidual cells appearing during normal murine pregnancy was investigated in semiallogeneic hemopoietic chimeras with retained or regained fertility. Chimeras were produced by three different methods in two donor-host combinations: F1 [BALB/c female x C3H/HeJ male] cells introduced into the parental strain BALB/c female hosts or F1 [CBA/J female x C57BL/6 male] cells introduced into CBA/J female hosts. Prenatal chimeras (PN) were made by reconstituting mouse fetuses (day 13-17) with 10(6)-10(7) adult bone marrow or fetal liver cells through the yolk sac and they were allowed to be delivered naturally. Neonatal chimeras (NN) were made by injecting 1-2 x 10(7) adult bone marrow cells into the anterior facial vein of neonatal mice (less than 24 hr old). In both cases, experimental animals were raised to maturity. Ovary-transplanted chimeras (OT) were made by injecting 10(7) bone marrow cells into lethally irradiated (9.5 Gy) young adult female mice, followed 6 weeks later with bilateral orthotopic transplants of syngeneic ovary grafts to restore fertility. All female chimeras produced by the three different methods were mated with syngeneic male partners to produce normal pregnancy. The extent of chimerism at the cellular level was determined in all cases by a radioautographic identification of the H-2 phenotype of splenic lymphocytes and decidual cells and macrophages in the collagenase-dispersed decidua at day 11-16 of normal pregnancy, following a sandwich labelling with monospecific anti-H-2 antibodies and 125I-protein A. Morphological discrimination of typical decidual cells from macrophages in the collagenase-dispersed decidua was carried out on the basis of several distinctive markers: presence of surface Dec-1 and Thy-1 and absence of surface F4/80 or latex phagocytosis for decidual cells, in contrast to macrophages which were phagocytic and expressed F4/80 but not Dec-1 or Thy-1. While the degree of hemopoietic chimerism (judged by the incidence of donor-derived lymphocytes in the spleen) varied from animal to animal, in all three groups (PN, NN, and OT) comprising a total of 26 chimeras, the percentage of typical decidual cells expressing donor H-2 phenotype showed an excellent correlation with that for small lymphocytes in the spleen. These results reveal that at least a subpopulation of typical decidual cells of the pregnant uterus has a hemopoietic genealogy. A possible familial relationship of these cells to granulated metrial gland cells remains unclear.
在保留或恢复生育能力的半同种异体造血嵌合体中，研究了正常小鼠妊娠期间出现的某些子宫蜕膜细胞前体可能的造血起源。通过三种不同方法在两种供体-宿主组合中产生嵌合体：将F1 [BALB/c雌性×C3H/HeJ雄性]细胞引入亲本品系BALB/c雌性宿主，或将F1 [CBA/J雌性×C57BL/6雄性]细胞引入CBA/J雌性宿主。产前嵌合体（PN）通过经卵黄囊用10(6)-10(7)个成年骨髓或胎儿肝细胞重建小鼠胎儿（第13 - 17天）制成，然后让它们自然分娩。新生嵌合体（NN）通过将1 - 2×10(7)个成年骨髓细胞注入新生小鼠（小于24小时龄）的面静脉前部制成。在这两种情况下，实验动物均饲养至成熟。卵巢移植嵌合体（OT）通过将10(7)个骨髓细胞注入经致死性照射（9.5 Gy）的年轻成年雌性小鼠制成，6周后进行双侧原位移植同基因卵巢移植物以恢复生育能力。通过三种不同方法产生的所有雌性嵌合体均与同基因雄性配偶交配以产生正常妊娠。在正常妊娠第11 - 16天，通过用单特异性抗H - 2抗体和125I - 蛋白A进行夹心标记后，通过放射自显影鉴定脾淋巴细胞以及胶原酶分散的蜕膜中的蜕膜细胞和巨噬细胞的H - 2表型，在所有情况下确定细胞水平的嵌合程度。基于几种独特的标志物对胶原酶分散的蜕膜中的典型蜕膜细胞和巨噬细胞进行形态学鉴别：蜕膜细胞表面存在Dec - 1和Thy - 1，且不存在表面F4/80或乳胶吞噬作用，与之相反，巨噬细胞具有吞噬作用且表达F4/80，但不表达Dec - 1或Thy - 1。虽然造血嵌合程度（根据脾中供体来源淋巴细胞的发生率判断）因动物而异，但在总共26只嵌合体的所有三组（PN、NN和OT）中，表达供体H - 2表型的典型蜕膜细胞百分比与脾中小淋巴细胞的百分比显示出极好的相关性。这些结果表明，妊娠子宫中至少有一部分典型蜕膜细胞具有造血谱系。这些细胞与颗粒状子宫内膜腺细胞之间可能的家族关系仍不清楚。
