Brody J R, Cunha G R
Department of Anatomy, University of California, San Francisco 94143.
Am J Anat. 1989 Sep;186(1):1-20. doi: 10.1002/aja.1001860102.
Myometrial development from the prenatal to adult period was examined in rats and mice 1) by histologic and immunocytochemical methods with anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells; and 2) by morphometric procedures to assess quantitatively the expression of cellular orientation in the emerging inner circular myometrial layer. Uterine mesenchymal cells initially were uniformly vimentin-positive, undifferentiated, and randomly oriented during the late fetal period. By the early neonatal period, three mesenchymal layers became recognizable histologically, the middle one of which (prospective circular myometrium) developed distinct circular orientation and differentiated into a layer composed of actin-positive smooth muscle cells. The cells of the inner mesenchymal layer initially exhibited radial orientation. By 10 days postpartum, the outer longitudinal mesenchymal layer differentiated into bundles of smooth muscle cells representing the longitudinal myometrium. The inner mesenchymal layer remained vimentin-positive and differentiated into the randomly ordered endometrial stroma. The cells of the middle and outer mesenchymal layers that were destined to form myometrium initially expressed vimentin throughout and then coexpressed vimentin and actin, but with time vimentin staining disappeared in the maturing smooth muscle cells as they expressed actin.
1）采用组织学和免疫细胞化学方法，使用抗肌动蛋白、波形蛋白和层粘连蛋白抗体，以评估平滑肌细胞和成纤维细胞的细胞分化；2）采用形态计量学方法，定量评估新生内环子宫肌层中细胞取向的表达。在胎儿后期，子宫间充质细胞最初均为波形蛋白阳性、未分化且随机取向。到新生儿早期，在组织学上可识别出三层间充质，其中中间一层（未来的环形子宫肌层）形成明显的环形取向，并分化为由肌动蛋白阳性平滑肌细胞组成的一层。内侧间充质层的细胞最初呈放射状取向。产后10天，外侧纵行间充质层分化为代表纵行子宫肌层的平滑肌细胞束。内侧间充质层仍为波形蛋白阳性，并分化为随机排列的子宫内膜基质。注定要形成子宫肌层的中间和外侧间充质层细胞最初全程表达波形蛋白，然后同时表达波形蛋白和肌动蛋白，但随着时间推移，随着成熟平滑肌细胞表达肌动蛋白，波形蛋白染色消失。
