Yao Qianqian, Liang Hengxing, Huang Bo, Xiang Lin, Wang Tianlu, Xiong Yi, Yang Bo, Guo Yanjun, Gong Ping
Oral Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.
Department of Thoracic Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.
Biochem Biophys Res Commun. 2017 Jul 8;488(4):634-640. doi: 10.1016/j.bbrc.2016.11.011. Epub 2016 Nov 5.
The sympathetic nervous system play a pivotal role in bone remodeling through β-adrenoceptor (β-AR). However, it is not well documented whether the β-adrenoceptor pathway has the potential to influence osteocytes. In this study, cell viability, the expression of β-AR subtypes, enzymes of catecholamine synthesis or degradation, bone-related gene and protein in osteocytic MLO-Y4 cells were investigated by β-adrenergic stimulation. Isoproterenol (ISO) promoted RANKL to OPG expression in osteocytes, as well as osteoclasts formation in osteocytes-RAW264.7 cell co-cultures but not RAW264.7 cell monoculture. The ISO-stimulated effect was enhanced in β1-AR antagonist pretreatment, but was rescued by blocking β2-AR. The results indicate that β1-and β2-AR play reciprocal roles on MLO-Y4 cells in the regulation of osteoclastogenesis, and osteocyte β-adrenergic signaling might be a new valuable therapy for bone disease.
交感神经系统通过β-肾上腺素能受体（β-AR）在骨重塑中起关键作用。然而，β-肾上腺素能受体途径是否有影响骨细胞的潜力，目前尚无充分的文献记载。在本研究中，通过β-肾上腺素能刺激，研究了骨细胞系MLO-Y4细胞的细胞活力、β-AR亚型的表达、儿茶酚胺合成或降解的酶、骨相关基因和蛋白质。异丙肾上腺素（ISO）促进了骨细胞中RANKL向OPG的表达，以及骨细胞与RAW264.7细胞共培养体系中破骨细胞的形成，但在RAW264.7细胞单培养体系中未出现此现象。β1-AR拮抗剂预处理增强了ISO刺激的效果，但通过阻断β2-AR可使其恢复。结果表明，β1-AR和β2-AR在调节破骨细胞生成方面对MLO-Y4细胞发挥相反作用，并且骨细胞β-肾上腺素能信号传导可能是治疗骨疾病的一种新的有价值的方法。
