用于定量瘤胃细菌对纤维素黏附性的改良测定法。
Improved assay for quantitating adherence of ruminal bacteria to cellulose.
作者信息
Rasmussen M A, White B A, Hespell R B
机构信息
Department of Animal Sciences, University of Illinois, Urbana 61801.
出版信息
Appl Environ Microbiol. 1989 Aug;55(8):2089-91. doi: 10.1128/aem.55.8.2089-2091.1989.
Abstract
A quantitative technique suitable for the determination of adherence of ruminal bacteria to cellulose was developed. This technique employs adherence of cells to cellulose disks and alleviates the problem of nonspecific cell entrapment within cellulose particles. By using this technique, it was demonstrated that the adherence of Ruminococcus flavefaciens FD1 to cellulose was inhibited by formaldehyde, methylcellulose, and carboxymethyl cellulose. Adherence was unaffected by acid hydrolysates of methylcellulose, glucose, and cellobiose.
摘要
开发了一种适用于测定瘤胃细菌对纤维素黏附性的定量技术。该技术利用细胞对纤维素圆盘的黏附，解决了纤维素颗粒中非特异性细胞截留的问题。通过使用该技术，证明了黄化瘤胃球菌FD1对纤维素的黏附受到甲醛、甲基纤维素和羧甲基纤维素的抑制。黏附不受甲基纤维素、葡萄糖和纤维二糖的酸水解产物的影响。
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