Lamed R, Naimark J, Morgenstern E, Bayer E A
J Bacteriol. 1987 Aug;169(8):3792-800. doi: 10.1128/jb.169.8.3792-3800.1987.
The cell surface topology of various gram-negative and -positive, anaerobic and aerobic, mesophilic and thermophilic, cellulolytic and noncellulolytic bacteria was investigated by scanning electron microscopic visualization using cationized ferritin. Characteristic protuberant structures were observed on cells of all cellulolytic strains. These structures appeared to be directly related to the previously described exocellular cellulase-containing polycellulosomes of Clostridium thermocellum YS (E. A. Bayer and R. Lamed, J. Bacteriol. 167:828-836, 1986). Immunochemical evidence and lectin-binding studies suggested a further correlation on the molecular level among cellulolytic bacteria. The results indicate that such cell surface cellulase-containing structures may be of general consequence to the bacterial interaction with and degradation of cellulose.
利用阳离子铁蛋白通过扫描电子显微镜可视化技术，对各种革兰氏阴性菌和阳性菌、厌氧菌和好氧菌、嗜温菌和嗜热菌、纤维素分解菌和非纤维素分解菌的细胞表面拓扑结构进行了研究。在所有纤维素分解菌株的细胞上均观察到特征性的突出结构。这些结构似乎与先前描述的热纤梭菌YS含细胞外纤维素酶的多纤维素体直接相关（E.A.拜尔和R.拉梅德，《细菌学杂志》167:828 - 836，1986年）。免疫化学证据和凝集素结合研究表明，在纤维素分解菌之间的分子水平上存在进一步的相关性。结果表明，这种含细胞表面纤维素酶的结构可能对细菌与纤维素的相互作用及纤维素降解具有普遍意义。
