Ben Mansour Rim, Ksouri Wided Megdiche, Cluzet Stéphanie, Krisa Stéphanie, Richard Tristan, Ksouri Riadh
Laboratory of Aromatic and Medicinal Plants, Center of Biotechnology, Technopark of Borj-Cedria (CBBC), BP 901, 2050 Hammam-Lif, Tunisia.
University of Bordeaux, ISVV, GESVAB EA3675, 33882 Villenave d'Ornon, France.
Evid Based Complement Alternat Med. 2016;2016:2843463. doi: 10.1155/2016/2843463. Epub 2016 Oct 18.
This work aimed to investigate the richness of a Tunisian xerohalophyte aerial and root parts on phenolics and to evaluate the antioxidant and neuroprotective properties of this medicinal species. After fractionation using increasing and different solvent polarities, results displayed five fractions, where ethyl acetate (EtOAc) shoot and root fractions possess considerable total phenolic contents (221 and 308 mg of GAE/g of E, resp.) related to their important antioxidant activities such as ORAC (918 and 713 mg of TE/g of E), DPPH (282 and 821 mg of TE/g), and ABTS (778 and 1320 mg of TE/g) tests. Then, the identification of the main compounds by HPLC-DAD-ESI-MS and neuroprotective property of the most active fraction EtOAc were assessed. A total of 14 molecules were identified, which have been described for the first time in . The major compounds identified were pinoresinol and kaempferol glycoside in aerial parts and gallic acid and ellagitannin in roots. Neuroprotective capacity against -amyloid (A) peptide induced toxicity in PC12 cells of EtOAc fraction showed a significant protective activity at lower concentration (25 and 50 M). The relevant antioxidant and neuroprotective activities of EtOAc fraction corroborated their chemical compositions.
本研究旨在调查突尼斯旱生盐生植物地上部分和根部酚类物质的丰富程度，并评估该药用植物的抗氧化和神经保护特性。在使用极性递增且不同的溶剂进行分级分离后，结果显示有五个级分，其中乙酸乙酯（EtOAc）地上部分和根部级分具有相当高的总酚含量（分别为221和308毫克没食子酸当量/克提取物），这与其重要的抗氧化活性相关，如氧自由基吸收能力（ORAC）测试（918和713毫克Trolox当量/克提取物）、二苯基苦味酰基自由基（DPPH）测试（282和821毫克Trolox当量/克）以及2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）测试（778和1320毫克Trolox当量/克）。然后，通过高效液相色谱-二极管阵列检测器-电喷雾电离质谱（HPLC-DAD-ESI-MS）对主要化合物进行鉴定，并评估活性最高的级分EtOAc的神经保护特性。总共鉴定出14种分子，这些分子在……中首次被描述。在地上部分鉴定出的主要化合物是松脂醇和山奈酚糖苷，在根部是没食子酸和鞣花单宁。EtOAc级分对β-淀粉样蛋白（Aβ）肽诱导的PC12细胞毒性的神经保护能力在较低浓度（25和50μM）时显示出显著的保护活性。EtOAc级分相关的抗氧化和神经保护活性证实了它们的化学组成。
