LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France.
LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France.
J Photochem Photobiol B. 2015 Feb;143:52-60. doi: 10.1016/j.jphotobiol.2014.12.018. Epub 2014 Dec 27.
In temperate saltmarshes, halophytic plants have to daily protect their internal tissues against sunlight and UV rays. Consequently, they develop adaptive responses such as the synthesis of secondary metabolites, including polyphenols. The present study focused on the biological activities of fractions enriched in polyphenols from Salicornia ramosissima. Three different extracts were obtained by purification processes to concentrate polyphenols: a crude hydroalcoholic extract, and two purified fractions: an ethyl acetate fraction (EAF) and an aqueous fraction. Phenolic and flavonoid contents, antioxidant (DPPH radical-scavenging activity, reducing activity, β-carotene linoleic acid system and the ORAC method) and sunscreen properties (Sun Protection Factor and UVA-Protection Factor) were assessed by in vitro tests. The purification process was effective in increasing phenolic and flavonoid contents as well as antioxidant and sunscreen capacities of the EAF. The EAF appeared to be a broad spectrum UV absorber. The chemical structure of 10 EAF polyphenols was elucidated using 2D NMR and mass spectrometry spectra. Furthermore, a correlation was observed between phenolic composition and biological activity. These findings are encouraging for the future use of S. ramosissima as a potential source of antioxidant and photoprotectant molecules for industrial applications.
在温带盐沼中，盐生植物必须每天保护其内部组织免受阳光和紫外线的伤害。因此，它们会产生适应性反应，如合成包括多酚在内的次生代谢物。本研究集中于从盐角草中富含多酚的馏分的生物活性。通过纯化过程获得了三种不同的多酚浓缩提取物：粗水醇提取物和两种纯化馏分：乙酸乙酯馏分（EAF）和水馏分。通过体外试验评估了多酚和类黄酮含量、抗氧化（DPPH 自由基清除活性、还原活性、β-胡萝卜素亚油酸系统和 ORAC 方法）和防晒性能（防晒因子和 UVA 保护因子）。纯化过程有效地提高了 EAF 的多酚和类黄酮含量以及抗氧化和防晒能力。EAF 似乎是一种广谱的紫外线吸收剂。使用 2D NMR 和质谱谱图阐明了 10 种 EAF 多酚的化学结构。此外，还观察到了酚类成分与生物活性之间的相关性。这些发现为未来将盐角草用作工业应用中抗氧化和光保护分子的潜在来源提供了希望。
