Gaillard Martin, Dagher Ibrahim
INSERM U1193, Paul Brousse Hospital, Villejuif, F-91807, France.
Department of Digestive Minimally Invasive Surgery, Antoine Béclère Hospital, AP-HP, Clamart, F-92140, France.
Methods Mol Biol. 2017;1506:193-200. doi: 10.1007/978-1-4939-6506-9_13.
In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.
在肝实质细胞移植的背景下，预处理对于增强细胞植入和肝脏再填充至关重要。作者开发了一种使用可吸收材料进行临时门静脉栓塞的微创技术，称为可逆门静脉栓塞。在此，我们描述了在受体进行可逆门静脉栓塞后移植肝细胞之前，从供体大鼠分离肝细胞的方法。
