INSERM U1193, Hôpital Paul-Brousse, Villejuif, France.
Département Hospitalo-Universitaire Hepatinov, Hôpital Paul-Brousse, Villejuif, France.
Liver Transpl. 2019 Jan;25(1):98-110. doi: 10.1002/lt.25364.
Hepatocyte transplantation (HT) has emerged as a promising alternative to orthotopic liver transplantation, yet liver preconditioning is needed to promote hepatocyte engraftment. A method of temporary occlusion of the portal flow called reversible portal vein embolization (RPVE) has been demonstrated to be an efficient method of liver preconditioning. By providing an additional regenerative stimulus, repeated reversible portal vein embolization (RRPVE) could further boost liver engraftment. The aim of this study was to determine the efficiency of liver engraftment of transplanted hepatocytes after RPVE and RRPVE in a rat model. Green fluorescent protein-expressing hepatocytes were isolated from transgenic rats and transplanted into 3 groups of syngeneic recipient rats. HT was associated with RPVE in group 1, with RRPVE in group 2, and with sham embolization in the sham group. Liver engraftment was assessed at day 28 after HT on liver samples after immunostaining. Procedures were well tolerated in all groups. RRPVE resulted in increased engraftment rate in total liver parenchyma compared with RPVE (3.4% ± 0.81% versus 1.4% ± 0.34%; P < 0.001). In conclusion, RRPVE successfully enhanced hepatocyte engraftment after HT and could be helpful in the frame of failure of HT due to low cell engraftment.
肝细胞移植（HT）已成为肝移植的一种有前途的替代方法，但需要进行肝预处理以促进肝细胞植入。一种称为可复性门静脉栓塞（RPVE）的门静脉临时阻断方法已被证明是一种有效的肝预处理方法。通过提供额外的再生刺激，重复可复性门静脉栓塞（RRPVE）可以进一步促进肝植入。本研究旨在确定在大鼠模型中 RPVE 和 RRPVE 后移植肝细胞的肝植入效率。从转基因大鼠中分离出表达绿色荧光蛋白的肝细胞，并将其移植到 3 组同基因受体大鼠中。HT 与第 1 组的 RPVE 相关，与第 2 组的 RRPVE 相关，与 sham 组的 sham 栓塞相关。在 HT 后 28 天，通过免疫染色评估肝样本中的肝植入情况。所有组的程序均耐受良好。RRPVE 导致总肝实质中的植入率高于 RPVE（3.4%±0.81%比 1.4%±0.34%；P<0.001）。总之，RRPVE 成功地增强了 HT 后的肝细胞植入，并且在由于细胞植入率低而导致 HT 失败的情况下可能会有所帮助。
