Olicón-Hernández Dario Rafael, Vázquez-Landaverde Pedro Alberto, Cruz-Camarillo Ramón, Rojas-Avelizapa Luz Irene
a Department of Microbiology, ENCB-IPN, Col Santo Tomas , Delegación Miguel Hidalgo , México.
b CICATA-IPN Querétaro, Col Colinas del Cimatario , Querétaro , México.
Prep Biochem Biotechnol. 2017 Feb 7;47(2):116-122. doi: 10.1080/10826068.2016.1181086. Epub 2016 Nov 10.
Bacillus thuringiensis is a nonhuman pathogen bacterium that is used as a fungal and insect biocontrol agent. Because of its environmental interaction, it possesses several extracellular enzymes that are able to degrade chitin and chitosan, two of the most important polymers because of their application in numerous fields. However, in recent years, it has been observed that oligosaccharides from the enzymatic degradation of chitosan have important benefits for human health. Comparison and exploration of the production of chito-oligosaccharides from different sources of chitosan will improve the process parameters and expand the biotechnology based in these molecules. This study shows the production of chito-oligosaccharides from three different sources of colloidal chitosan and conducts a qualitative-quantitative comparison between them, using the extracellular enzyme of B. thuringiensis. We found that in the three substrates, it is possible to get a mixture of chito-oligosaccharides from dimer to hexamer in a concentration range from 0.72 to 8.09 mg · g of original substrate. The best substrate to obtain these molecules was commercial chitosan as it has the highest production yields.
苏云金芽孢杆菌是一种非人类病原菌，用作真菌和昆虫生物防治剂。由于其与环境的相互作用，它拥有几种能够降解几丁质和壳聚糖的胞外酶，这两种聚合物因其在众多领域的应用而成为最重要的聚合物。然而，近年来，人们观察到壳聚糖酶促降解产生的低聚糖对人体健康有重要益处。比较和探索从不同壳聚糖来源生产壳寡糖将改善工艺参数，并扩展基于这些分子的生物技术。本研究展示了利用苏云金芽孢杆菌的胞外酶从三种不同来源的胶体壳聚糖生产壳寡糖，并对它们进行了定性和定量比较。我们发现，在这三种底物中，有可能获得从二聚体到六聚体的壳寡糖混合物，其浓度范围为每克原始底物0.72至8.09毫克。获得这些分子的最佳底物是商业壳聚糖，因为它的产量最高。
