Guillot Raúl, Muriach Borja, Rocha Ana, Rotllant Josep, Kelsh Robert N, Cerdá-Reverter José Miguel
Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre de la Sal, Consejo Superior de Investigaciones Científicas, (IATS-CSIC), Ribera de Cabanes, Castellón, Spain, 12595.
Facultad Ciencias de la Salud, Universidad CEU Cardenal Herrera, Castellón, Spain, 12006.
PLoS One. 2016 Nov 10;11(11):e0166152. doi: 10.1371/journal.pone.0166152. eCollection 2016.
Zebrafish embryos are treated with anti-thyroidal compounds, such as phenylthiourea, to inhibit melanogenesis. However, the mechanism whereby the thyroidal system controls melanin synthesis has not been assessed in detail. In this work, we tested the effect of the administration of diets supplemented with T3 (500μg/g food) on the pigment pattern of adult zebrafish. Oral T3 induced a pronounced skin paling in both adult female and male zebrafish that was reversible upon cessation of treatment. The number of visible melanophores was significantly reduced in treated fish. Accordingly, treatment down-regulated expression of tyrosinase-related protein 1 in both sexes. We also found sexually dimorphic regulation of some melanogenic genes, such as Dct/Tyrp2 that was dramatically up-regulated in females after T3 treatment. Thus, we demonstrated that melanogenesis is reversibly inhibited by thyroid hormones in adult zebrafish and make the discovery of gender-specific differences in the response of melanogenic gene expression. Thus, fish gender is now shown to be an important variable that should be controlled in future studies of fish melanogenesis.
斑马鱼胚胎用抗甲状腺化合物如苯硫脲处理，以抑制黑色素生成。然而，甲状腺系统控制黑色素合成的机制尚未得到详细评估。在这项工作中，我们测试了在成年斑马鱼的食物中添加T3（500μg/g食物）对其色素模式的影响。口服T3导致成年雌性和雄性斑马鱼皮肤明显变白，停止治疗后这种变化是可逆的。在接受处理的鱼中，可见黑素细胞的数量显著减少。相应地，处理下调了两性中酪氨酸酶相关蛋白1的表达。我们还发现了一些黑色素生成基因的性别二态性调控，例如Dct/Tyrp2在T3处理后的雌性中显著上调。因此，我们证明了成年斑马鱼的黑色素生成受到甲状腺激素的可逆抑制，并发现了黑色素生成基因表达反应中的性别特异性差异。因此，现在表明鱼类性别是未来鱼类黑色素生成研究中应加以控制的一个重要变量。
