Yu Songlin, Zhou Weiyan, Cheng Xinqi, Fang Huiling, Zhang Ruiping, Cheng Qian, Han Jianhua, Su Wei, Xia Liangyu, Qiu Ling
Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, 100730, China.
Beijing Hospital National Center for Clinical Laboratories, Ministry of Health, Beijing, 100730, China.
PLoS One. 2016 Nov 10;11(11):e0166327. doi: 10.1371/journal.pone.0166327. eCollection 2016.
A significant bias was found when using the Siemens ADVIA Centaur XP system for measurement of 25-hydroxyvitamin D (25OHD) with VACUETTE® tubes with Serum Clot Activator and Gel. Here, we examined whether other commonly used tubes or temperatures affected 25OHD results obtained with the Siemens ADVIA Centaur XP system. Serum was collected into five types of vacuum blood collection tubes from three manufacturers, and 25OHD was analyzed using the Siemens ADVIA Centaur XP system and liquid chromatography tandem mass spectrometry (LC-MS/MS) immediately or after storage at 4°C or -80°C for 48 h. Significantly higher 25OHD values were found when using the Siemens ADVIA Centaur XP system with VACUETTE® tubes with serum clot activator and gel and VACUETTE® tubes with clot activator but no gel compared with VACUETTE® tubes with no additives. The 25OHD values in all of these tubes were not significantly different from those obtained by LC-MS/MS. Moreover, after storage at -80°C for 48 h, the values obtained in IMPROVEVACUTER® tubes with serum clot activator and gel significantly increased, with a mean bias of 74.6% compared with the values before storage, on analysis with the Siemens ADVIA Centaur XP system. VACUETTE® tubes containing additives significantly affect the accuracy of 25OHD results obtained using the Siemens ADVIA Centaur XP system. Additionally, the composition of serum collected in IMPROVEVACUTER® tubes was affected by freezing, resulting in different measurements when using the Siemens 25OHD assay platform.
在使用西门子ADVIA Centaur XP系统，采用含有血清促凝剂和凝胶的VACUETTE®管测定25-羟基维生素D（25OHD）时，发现存在显著偏差。在此，我们研究了其他常用试管或温度是否会影响使用西门子ADVIA Centaur XP系统获得的25OHD检测结果。将血清收集到来自三个制造商的五种真空采血管中，立即或在4°C或-80°C储存48小时后，使用西门子ADVIA Centaur XP系统和液相色谱串联质谱法（LC-MS/MS）分析25OHD。与无添加剂的VACUETTE®管相比，使用含有血清促凝剂和凝胶的VACUETTE®管以及含有促凝剂但无凝胶的VACUETTE®管的西门子ADVIA Centaur XP系统时，发现25OHD值显著更高。所有这些试管中的25OHD值与通过LC-MS/MS获得的值无显著差异。此外，在-80°C储存48小时后，使用西门子ADVIA Centaur XP系统分析时，含有血清促凝剂和凝胶的IMPROVEVACUTER®管中获得的值显著增加，与储存前的值相比，平均偏差为74.6%。含有添加剂的VACUETTE®管会显著影响使用西门子ADVIA Centaur XP系统获得的25OHD检测结果的准确性。此外，收集在IMPROVEVACUTER®管中的血清成分受冷冻影响，导致使用西门子25OHD检测平台时测量结果不同。
