Liu X, Ma M, Duan X, Zhang H, Yang M
Key Laboratory of Cancer Prevention and Therapy, The Second Department of Thoracic Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin's Clinical Research Center for Cancer, National Clinical Research Center for Cancer, Huanhuxi Road, Hexi District, Tianjin, 300060, China.
Clin Transl Oncol. 2017 May;19(5):587-592. doi: 10.1007/s12094-016-1569-y. Epub 2016 Nov 10.
Cisplatin is commonly used in non-small-cell lung cancer (NSCLC) chemotherapy; however, chemoresistance to cisplatin remains a great clinical challenge. Octamer-binding protein 4 (OCT4) has been reported to be overexpressed in NSCLC. In this study, we aimed to investigate the potential role of OCT4 in NSCLC with chemoresistance to cisplatin.
Expressions of OCT4 was detected in NSCLC tissues and cell lines. We utilized siRNA to knock down OCT4 expression in human NSCLC cells and analyzed their phenotypic changes.
We found that the difference of OCT4 expression between NSCLC and the adjacent non-tumourous tissues was statistically significant. Knockdown of OCT4 in NSCLC cells could decrease cell proliferation, and potentiate apoptosis induced by cisplatin, suggesting OCT4 may contribute to cisplatin resistance in NSCLC.
Our findings indicate that targeting OCT4 could improve cisplatin effect in NSCLC, confirming their role in modulating cisplatin sensitivity.
顺铂常用于非小细胞肺癌（NSCLC）化疗；然而，对顺铂的化疗耐药仍然是一个巨大的临床挑战。据报道，八聚体结合蛋白4（OCT4）在NSCLC中过表达。在本研究中，我们旨在探讨OCT4在对顺铂耐药的NSCLC中的潜在作用。
检测NSCLC组织和细胞系中OCT4的表达。我们利用小干扰RNA（siRNA）敲低人NSCLC细胞中OCT4的表达，并分析其表型变化。
我们发现NSCLC与相邻非肿瘤组织中OCT4表达的差异具有统计学意义。敲低NSCLC细胞中的OCT4可降低细胞增殖，并增强顺铂诱导的细胞凋亡，提示OCT4可能导致NSCLC对顺铂耐药。
我们的研究结果表明，靶向OCT4可提高顺铂在NSCLC中的疗效，证实了其在调节顺铂敏感性中的作用。
