Sechman Andrzej, Batoryna Marta, Antos Piotr A, Hrabia Anna
Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland.
Department of Animal Physiology and Endocrinology, University of Agriculture in Krakow, Al. Mickiewicza 24/28, 30-059 Krakow, Poland.
Toxicol Lett. 2016 Dec 15;264:29-37. doi: 10.1016/j.toxlet.2016.11.001. Epub 2016 Nov 8.
The objective of this study was to assess the in vitro effects of dioxin-like PCB 126 and non-dioxin-like PCB 153 on basal and ovine LH (oLH)-stimulated testosterone (T) and estradiol (E2) secretion and expression of steroidogenic genes (STAR, HSD3B and CYP19A1) and estrogen receptors α (ERα) and β (ERβ) in white (WF) and yellowish (YF) prehierarchical follicles of the hen ovary. Steroid concentrations in a medium and gene expression in follicles following 6h of exposition were determined by RIA and real-time qPCR, respectively. Both PCBs increased basal and oLH-stimulated T secretion by the WF follicles. PCB 126 reduced basal E2 secretion by the WF follicles. PCB 153 elevated but PCB 126 reduced oLH-stimulated E2 secretion by the prehierarchical follicles. PCB 126 increased basal STAR and HSD3B and reduced CYP19A1 mRNA expression in these follicles. PCB 153 increased basal expression of STAR and HSD3B in YF follicles, but diminished HSD3B mRNA levels in the WF. The studied PCBs had an opposite effect on basal and oLH-stimulated CYP19A1 mRNA expression in prehierarchical follicles. Both PCBs modulated basal and inhibited oLH-stimulated ERα and ERβ gene expression in the prehierarchical follicles. In conclusion, data of the current study demonstrate the congener-specific effects of PCBs on sex steroid secretion by prehierarchical follicles of the chicken ovary, which are at least partly related to STAR, HSD3B and CYP19A1 gene expression. It is suggested that PCBs, by influencing follicular steroidogenesis and expression of estrogen receptors, may impair development and selection of yellowish follicles to the preovulatory hierarchy.
本研究的目的是评估二噁英类多氯联苯126和非二噁英类多氯联苯153对母鸡卵巢白色（WF）和淡黄色（YF）分级前卵泡基础状态下以及绵羊促黄体生成素（oLH）刺激下的睾酮（T）和雌二醇（E2）分泌、类固醇生成基因（STAR、HSD3B和CYP19A1）以及雌激素受体α（ERα）和β（ERβ）表达的体外影响。分别通过放射免疫分析（RIA）和实时定量聚合酶链反应（qPCR）测定暴露6小时后培养基中的类固醇浓度和卵泡中的基因表达。两种多氯联苯均增加了WF卵泡基础状态下以及oLH刺激下的T分泌。多氯联苯126降低了WF卵泡基础状态下的E2分泌。多氯联苯153提高了分级前卵泡oLH刺激下的E2分泌，但多氯联苯126降低了该分泌。多氯联苯126增加了这些卵泡基础状态下的STAR和HSD3B表达，并降低了CYP19A1 mRNA表达。多氯联苯153增加了YF卵泡基础状态下的STAR和HSD3B表达，但降低了WF卵泡中HSD3B mRNA水平。所研究的多氯联苯对分级前卵泡基础状态下以及oLH刺激下的CYP19A1 mRNA表达产生相反影响。两种多氯联苯均调节了分级前卵泡基础状态下的ERα和ERβ基因表达，并抑制了oLH刺激下的该基因表达。总之，本研究数据证明了多氯联苯对鸡卵巢分级前卵泡性类固醇分泌的同系物特异性影响，这至少部分与STAR、HSD3B和CYP19A1基因表达有关。提示多氯联苯通过影响卵泡类固醇生成和雌激素受体表达，可能损害淡黄色卵泡向排卵前等级的发育和选择。
