Xu Qianqian, Xu Yali, Pan Bo, Wu Liangcai, Ren Xinyu, Zhou Yidong, Mao Feng, Lin Yan, Guan Jinghong, Shen Songjie, Zhang Xiaohui, Wang Changjun, Zhong Ying, Zhou Liangrui, Liang Zhiyong, Zhao Haitao, Sun Qiang
Department of Breast Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Oncotarget. 2016 Dec 6;7(49):81815-81829. doi: 10.18632/oncotarget.13245.
Previous studies demonstrate that threonine and tyrosine kinase (TTK) is overexpressed in triple-negative breast cancer (TNBC), but there are conflicting results regarding its effect on TNBC survival. The purpose of this study was to assess the prognostic significance of TTK expression in primary TNBC.
Of 169 consecutive cases eligible for this study, 164 included follow-up information. Cytoplasm and membrane TTK staining was observed in 99.4% of cases, while 5.9% displayed whole cell immunostaining. At a discriminating threshold of 55, elevated TTK expression was associated with prolonged disease free survival (DFS) (p < 0.001) and overall survival (OS) (p = 0.024) in primary TNBC and prolonged DFS in individual basal-like (p = 0.001) and non-basal-like (p = 0.001) TNBC subtypes. In addition, Cox regression analysis demonstrated that elevated TTK expression was an independent prognostic factor for DFS in TNBC (p < 0.001).
TTK expression of 169 samples was tested by immunohistochemistry (IHC). A receiver operating characteristic (ROC) curve was used to identify a cutpoint for TTK expression, which was analyzed for its association with patients' clinicopathological factors and survival using Chi-square, log-rank, and Cox regression analyses.
TTK is a favorable prognostic biomarker associated with TNBC survival.
既往研究表明，苏氨酸和酪氨酸激酶（TTK）在三阴性乳腺癌（TNBC）中过表达，但关于其对TNBC生存的影响存在相互矛盾的结果。本研究的目的是评估TTK表达在原发性TNBC中的预后意义。
在本研究符合条件的169例连续病例中，164例包括随访信息。99.4%的病例观察到细胞质和膜TTK染色，而5.9%表现为全细胞免疫染色。在鉴别阈值为55时，原发性TNBC中TTK表达升高与无病生存期（DFS）延长（p<0.001）和总生存期（OS）延长（p=0.024）相关，在个体基底样（p=0.001）和非基底样（p=0.001）TNBC亚型中DFS延长。此外，Cox回归分析表明，TTK表达升高是TNBC中DFS的独立预后因素（p<0.001）。
采用免疫组织化学（IHC）检测169例样本的TTK表达。使用受试者工作特征（ROC）曲线确定TTK表达的切点，并使用卡方检验、对数秩检验和Cox回归分析分析其与患者临床病理因素和生存的相关性。
TTK是与TNBC生存相关的有利预后生物标志物。
