Wang Jingjing, Wang Lihao, Zhu Yinfang, Zhang Jinying, Liao Ju, Wang Shuaipeng, Yang Jinling, Yang Fuhua
Institute of Semiconductors, Chinese Academy of Sciences, Qinghua Donglu A 35, Beijing, 100083, People's Republic of China.
School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin, 300387, People's Republic of China.
Biomed Microdevices. 2016 Dec;18(6):110. doi: 10.1007/s10544-016-0132-5.
Early liver cancer diagnosis has clinical significance in treating cancer. Joint detection of multiple biomarkers has been considered as an effective and reliable method for early cancer diagnosis. In this work, a novel biosensor based on microcantilever array was batch-fabricated for multiple liver cancer biomarkers detection with high sensitivity, high accuracy, high throughput, and high specification. A micro-cavity was designed in the free end of the cantilever for local reaction between antibody and antigen, which can dramatically reduce the effect of adsorption-induced stiffness coefficient k variation. Furthermore,the pillar arrays in the micro-cavity were designed for increasing detection upper limit. A linear relationship between the relative frequency shift and the antigen concentration was observed for three liver cancer biomarkers, alpha-fetoprotein (AFP), γ-glutamyltranspeptidase II (GGT-2), and hepatocyte growth factor (HGF). Slight cross-reaction response to different antigens ensures high specificity of the sensor. These features will promote clinical application of the cantilever sensors in early cancer diagnosis.
早期肝癌诊断对癌症治疗具有临床意义。多种生物标志物的联合检测被认为是早期癌症诊断的一种有效且可靠的方法。在这项工作中，一种基于微悬臂梁阵列的新型生物传感器被批量制造出来，用于高灵敏度、高精度、高通量和高规格地检测多种肝癌生物标志物。在悬臂梁的自由端设计了一个微腔，用于抗体和抗原之间的局部反应，这可以显著降低吸附诱导的刚度系数k变化的影响。此外，微腔内的柱状阵列被设计用于提高检测上限。对于三种肝癌生物标志物，即甲胎蛋白（AFP）、γ-谷氨酰转肽酶II（GGT-2）和肝细胞生长因子（HGF），观察到相对频率偏移与抗原浓度之间存在线性关系。对不同抗原的轻微交叉反应确保了传感器的高特异性。这些特性将促进悬臂梁传感器在早期癌症诊断中的临床应用。
