微悬臂生物传感器检测耶尔森氏菌的增强结合效率。
Enhanced Binding Efficiency of Microcantilever Biosensor for the Detection of Yersinia.
机构信息
Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, Beijing 100049, China.
出版信息
Sensors (Basel). 2019 Jul 29;19(15):3326. doi: 10.3390/s19153326.
A novel microcantilever sensor was batch fabricated for Yersinia detection. The microcantilever surface modification method was optimized by introducing a secondary antibody to increase the number of binding sites. A novel microfluidic platform was designed and fabricated successfully. A 30 μL solution could fully react with the microcantilever surface. Those routines enhanced the binding efficiency between the target and receptor on the microcantilever. With this novel designed microfluidic platform, the specific adsorption of 10 Yersinia on the beam surface with modified F1 antibody was significantly enhanced.
一种新型的微悬臂传感器被批量制作用于检测耶尔森氏菌。通过引入二次抗体来增加结合位点的数量，优化了微悬臂表面的修饰方法。成功设计和制造了一种新型微流控平台。30μL 溶液可以与微悬臂表面完全反应。这些常规操作增强了目标和微悬臂表面上的受体之间的结合效率。使用这种新型设计的微流控平台，修饰后的 F1 抗体上的 10 个耶尔森氏菌的特异性吸附明显增强。
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