Zuo Haiyang, Zhang Junfang, Zhang Liuguang, Ren Xiaoxia, Chen Xiaoli, Hao Haisheng, Zhao Xueming, Wang Dong
The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.
JiLin Agriculture University Animal Science and Technology, Changchun, 130118, China.
PLoS One. 2016 Nov 11;11(11):e0164874. doi: 10.1371/journal.pone.0164874. eCollection 2016.
To explore variations in the transcription activity during spermiogenesis, round and elongated spermatids were collected from ICR/CD1 model mice using laser capture microdissection (LCM) and cauda epididymal sperm samples. The transcripts were sequenced using RNA-seq, and the reads were mapped to mm9. The majority of the reads (70%) in the round and elongated spermatids were mappable to known and predicted exons, but that in sperm was only 9%. The results of the distribution of reads suggested that alternative splicing was more complicated in sperm than in round and elongated spermatids. In the 19,127 genes, we detected the expression of 5,104 de novo genes and 91,112 alternative splicing events, and 12,105 were differentially expressed. Gene ontology (GO), InterPro domains, and KEGG revealed changes in gene transcription, mitochondrial protein translation, cellular components, and energy metabolism during spermiogenesis. The results provided considerable information about alternative splicing events, differentiallly expressed genes (DEGs), and novel transcriptions during spermiogenesis in mice.
为了探究精子发生过程中转录活性的变化，使用激光捕获显微切割技术（LCM）从ICR/CD1模型小鼠中收集圆形和长形精子细胞以及附睾尾部精子样本。使用RNA测序对转录本进行测序，并将读数映射到mm9。圆形和长形精子细胞中大部分读数（70%）可映射到已知和预测的外显子，但精子中的这一比例仅为9%。读数分布结果表明，精子中的可变剪接比圆形和长形精子细胞中的更为复杂。在19127个基因中，我们检测到5104个从头基因的表达和91112个可变剪接事件，其中12105个存在差异表达。基因本体论（GO）、InterPro结构域和KEGG分析揭示了精子发生过程中基因转录、线粒体蛋白质翻译、细胞成分和能量代谢的变化。这些结果提供了关于小鼠精子发生过程中可变剪接事件、差异表达基因（DEGs）和新转录本的大量信息。
