Berche P, Decreusefond C, Theodorou I, Stiffel C
Faculté de Médecine Necker-Enfants Malades, Laboratoire de Microbiologie, Paris, France.
J Immunol. 1989 Feb 1;142(3):932-9.
Two lines of mice genetically selected for high and low in vitro responses to PHA were used to evaluate the impact of T cell polyclonal expansion on acquired resistance to Listeria monocytogenes. The selective breeding induced two major consequences in low responder mice: (1) a reduction of the number of L3T4+ cells and (2) a restriction of T cell expansion upon PHA stimulation, predominantly affecting the Lyt-2+ subset, and associated with an abridgment of IL-2 production. In vivo PHA stimulation induced anti-Listeria protection in high responder mice, but was much less effective in low responder mice. Flow cytometer analysis revealed that T cell proliferation was also reduced in low responder mice during the course of Listeria infection, implying both L3T4+ and Lyt-2+ subsets. This defect did not apparently influence the kinetics of bacterial elimination in host tissues, which was similar in both lines during primary Listeria infection. In contrast, the expression of delayed-type hypersensitivity to Listeria antigens and the level of immunologic memory were significantly reduced in low responder mice. In vivo selective T cell depletion by anti-L3T4 or anti-Lyt-2 mAb allowed us to demonstrate the predominant role of Lyt-2+ cells in protection and that of L3T4+ cells in the expression of delayed-type hypersensitivity.
利用两组经基因选择、对PHA体外反应分别为高和低的小鼠，来评估T细胞多克隆扩增对获得性抗单核细胞增生李斯特菌能力的影响。选择性育种在低反应性小鼠中引发了两个主要后果：（1）L3T4+细胞数量减少；（2）PHA刺激后T细胞扩增受限，主要影响Lyt-2+亚群，并伴有IL-2产生减少。体内PHA刺激在高反应性小鼠中诱导出抗李斯特菌保护作用，但在低反应性小鼠中效果要差得多。流式细胞仪分析显示，在李斯特菌感染过程中，低反应性小鼠的T细胞增殖也减少，涉及L3T4+和Lyt-2+亚群。这种缺陷显然并未影响宿主组织中细菌清除的动力学，在原发性李斯特菌感染期间，两组小鼠的情况相似。相反，低反应性小鼠对李斯特菌抗原的迟发型超敏反应表达和免疫记忆水平显著降低。通过抗L3T4或抗Lyt-2单克隆抗体在体内选择性清除T细胞，使我们能够证明Lyt-2+细胞在保护中的主要作用以及L3T4+细胞在迟发型超敏反应表达中的主要作用。
