Czuprynski C J, Brown J F
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
Immunology. 1990 Sep;71(1):107-12.
Mice treated with purified anti-Lyt-2 monoclonal antibody (mAb) displayed a delayed ability to eliminate a primary Listeria monocytogenes infection from their spleens. Elimination of listeriae from the liver was unimpaired by anti-Lyt-2 mAb treatment. Treatment with anti-L3T4 mAb, alone or in combination with anti-Lyt-2 mAb, resulted in similar increases in the numbers of listeriae recovered from the spleens at 7 days after challenge. Listeria-infected mice that had been treated with anti-Lyt-2 mAb alone developed a strong delayed-type hypersensitivity (DTH) response, although it was significantly reduced as compared to control listeria-infected mice. In contrast, treatment with anti-L3T4 mAb severely impaired the development of DTH in listeria-infected mice. Treatment with anti-Lyt-2 mAb and anti-L3T4 mAb, singly or in combination, did not prevent mice from developing increased anti-listeria resistance if they were then immunized with a sublethal dose of L. monocytogenes. Treatment of mice with anti-Lyt-2 mAb or anti-L3T4 mAb before immunization, however, reduced the ability of their spleen cells to transfer anti-listeria resistance to recipient mice. These results indicate that Lyt-2+ cells make substantial contributions to the resistance of mice to primary L. monocytogenes infection, and to the ability of spleen cells from listeria-immunized mice to transfer resistance to naive recipients.
用纯化的抗Lyt-2单克隆抗体（mAb）处理的小鼠清除脾脏中初次感染的单核细胞增生李斯特菌的能力出现延迟。抗Lyt-2 mAb处理并未损害肝脏中李斯特菌的清除。单独使用抗L3T4 mAb或与抗Lyt-2 mAb联合使用，在攻击后7天从脾脏中回收的李斯特菌数量有相似的增加。单独用抗Lyt-2 mAb处理的感染李斯特菌的小鼠产生了强烈的迟发型超敏反应（DTH），尽管与对照感染李斯特菌的小鼠相比明显降低。相反，用抗L3T4 mAb处理严重损害了感染李斯特菌的小鼠中DTH的发展。单独或联合使用抗Lyt-2 mAb和抗L3T4 mAb处理，如果随后用亚致死剂量的单核细胞增生李斯特菌免疫，不会阻止小鼠产生增强的抗李斯特菌抗性。然而，在免疫前用抗Lyt-2 mAb或抗L3T4 mAb处理小鼠，会降低其脾细胞将抗李斯特菌抗性转移给受体小鼠的能力。这些结果表明，Lyt-2 +细胞对小鼠抵抗初次单核细胞增生李斯特菌感染以及对感染李斯特菌的小鼠脾细胞将抗性转移给未感染的受体的能力有重大贡献。
