Xool-Tamayo Jorge, Serrano-Gamboa Germán, Monforte-González Miriam, Mirón-López Gumersindo, Vázquez-Flota Felipe
Posgrado Interinstitucional en Ciencias Químicas y Bioquímicas, Facultad de Química, Universidad Autónoma de Yucatán, Calle 43 No. 613 x, Calle 90, Col. Inalámbrica, 97069, Mérida, Yucatán, México.
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Chuburná de Hidalgo, 97205, Mérida, Yucatán, México.
Biotechnol Lett. 2017 Feb;39(2):323-330. doi: 10.1007/s10529-016-2250-9. Epub 2016 Nov 11.
To analyze berberine and sanguinarine biosynthetic capacities of both in vitro shoot and root cultures of Argemone mexicana and tissues from entire plants at different developmental stages.
Berberine and sanguinarine were equally distributed in roots and aerial tissues of developing plantlet whereas, in juvenile plants, sanguinarine was only detected in roots. This alkaloid distribution was consistent with that of biosynthetic transcripts in juvenile plants. However, lower transcript abundance in plantlets´ leaves suggests that alkaloids were mainly formed in roots and then mobilized to this tissue. In vitro root cultures maintained similar alkaloid profiles to those from intact seedlings and plantlets. However, in addition to berberine, rootless shoot cultures accumulated high levels of sanguinarine and biosynthetic transcripts.
In vitro shoot cultures of A. mexicana can synthesize sanguinarine in addition to berberine. This represent a convenient system for the production of both alkaloids.
分析墨西哥刺蓟离体茎段和根培养物以及不同发育阶段全株组织中黄连素和血根碱的生物合成能力。
黄连素和血根碱在发育中的幼苗根和地上组织中分布均匀，而在幼龄植物中，仅在根中检测到血根碱。这种生物碱分布与幼龄植物中生物合成转录本的分布一致。然而，幼苗叶片中转录本丰度较低表明生物碱主要在根中形成，然后转运到该组织。离体根培养物保持与完整幼苗和幼苗相似的生物碱谱。然而，除黄连素外，无根茎培养物积累了高水平的血根碱和生物合成转录本。
墨西哥刺蓟离体茎培养物除了能合成黄连素外，还能合成血根碱。这是生产这两种生物碱的便利系统。
