ABC 转运蛋白 1，来自墨西哥天芥菜（罂粟科）种子的生物碱转运蛋白。

AmABCB1, an alkaloid transporter from seeds of Argemone mexicana L (Papaveraceae).

机构信息

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. Calle 43 No. 130 Chuburná 97205, Mérida, Yucatán, México.

Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada-ku, Kobe, 658-8558, Japan.

出版信息

Planta. 2021 Nov 16;254(6):122. doi: 10.1007/s00425-021-03780-4.

DOI:10.1007/s00425-021-03780-4

PMID:34786595

Abstract

An ABCB-type transporter for sanguinarine, a benzophenanthridine alkaloid, was isolated from Argemone mexicana seeds. An ABCB-type transporter, AmABCB1, was identified in a transcriptome from unfolding seedlings of A. mexicana by its amino acid sequence identity to previously characterized alkaloid transporters from Coptis japonica and Thalictrum minus. Expression analysis revealed mature seeds as its main location; meanwhile, in vitro assays in yeast cells showed that AmABCB1 had uptake and efflux activities for sanguinarine and berberine, respectively.

摘要

从墨西哥矢车菊种子中分离出一种 ABCB 型转运蛋白，用于血根碱，一种苯并菲啶生物碱。通过与已从日本黄连和少瓣鬼臼中鉴定出的生物碱转运蛋白的氨基酸序列同一性，从墨西哥矢车菊展开幼苗的转录组中鉴定出一种 ABCB 型转运蛋白，AmABCB1。表达分析表明成熟种子是其主要位置；同时，在酵母细胞中的体外测定表明，AmABCB1 分别对血根碱和小檗碱具有摄取和外排活性。