Guo Qing, Zhang Zhaohong, Song Youtao, Liu Shuo, Gao Wei, Qiao Heng, Guo Lili, Wang Jun
School of Environment, Liaoning University, Shenyang 110036, PR China.
School of Environment, Liaoning University, Shenyang 110036, PR China.
Chemosphere. 2017 Feb;168:599-605. doi: 10.1016/j.chemosphere.2016.11.019. Epub 2016 Nov 9.
In this study, the interaction between DNA and several cationic surfactants with different head groups such as ethyl hexadecyl dimethyl ammonium bromide (EHDAB), hexadecyl dimethyl benzyl ammonium chloride (HDBAC), and cetyl pyridinium bromide (CPB) were investigated by UV-vis absorption, fluorescence and circular dichroism (CD) spectroscopy, gel electrophoresis, and viscosity technologies. The results show that these cationic surfactants can interact with DNA and major binding modes are electrostatic and hydrophobic. Also, CPB and HDBAC molecules interact with DNA by partial intercalation, and CPB has slightly stronger intercalation than HDBAC, while EHDAB interacts with DNA by non-intercalation. The different head groups of the surfactant molecules can influence the interaction strength. CPB has the stronger interaction with DNA than the others. Moreover, surfactant concentration, the ratio of DNA and fluorescence probe, ionic strength can influence the interaction. The surfactants may interact with DNA by the competition reactions with BR for DNA-BR. The increase of ionic strength may favor the surface binding between DNA and surfactants to some extent. This work provides deep mechanistic insight on the toxicity of cationic surfactants with different head groups to DNA molecules.
在本研究中，通过紫外可见吸收光谱、荧光光谱、圆二色光谱、凝胶电泳和粘度技术，研究了DNA与几种具有不同头基的阳离子表面活性剂之间的相互作用，这些表面活性剂包括十六烷基二甲基乙基溴化铵（EHDAB）、十六烷基二甲基苄基氯化铵（HDBAC）和十六烷基溴化吡啶（CPB）。结果表明，这些阳离子表面活性剂可与DNA相互作用，主要结合模式为静电作用和疏水作用。此外，CPB和HDBAC分子通过部分插入与DNA相互作用，且CPB的插入作用略强于HDBAC，而EHDAB通过非插入方式与DNA相互作用。表面活性剂分子的不同头基可影响相互作用强度。CPB与DNA的相互作用比其他表面活性剂更强。此外，表面活性剂浓度、DNA与荧光探针的比例、离子强度均可影响相互作用。表面活性剂可能通过与DNA - BR竞争反应与DNA相互作用。离子强度的增加在一定程度上可能有利于DNA与表面活性剂之间的表面结合。这项工作为具有不同头基的阳离子表面活性剂对DNA分子的毒性提供了深入的机理见解。
