Gómez-Canela Cristian, Prats Eva, Piña Benjamí, Tauler Romà
Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona, Catalonia, Spain.
Centre d'Investigació I Desenvolupament, CID-CSIC, Jordi Girona 18-26, 08034 Barcelona, Catalonia, Spain.
Environ Pollut. 2017 Jan;220(Pt B):1231-1243. doi: 10.1016/j.envpol.2016.11.010. Epub 2016 Nov 11.
In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different exposure experiments. Analysis of Variance Simultaneous Component Analysis of changes on metabolite peak profile areas showed significant chlorpyrifos concentration and exposure time-dependent changes, clearly differentiating between exposed and non-exposed samples and between short (2 h) and long exposure times (6 h or 24 h). The changes observed in the concentrations of 50 muscle metabolites are indicative of induction of oxidative stress, of a general disruption of neurotransmitter metabolism, and of muscle exhaustion. These three effects are intimately related to the toxicity of chlorpyrifos.
在这项工作中，通过液相色谱与高分辨率质谱联用技术评估了毒死蜱暴露对斑马鱼肌肉代谢谱的影响。提出了基于感兴趣区域选择和多元曲线分辨交替最小二乘法的不同化学计量学工具，用于分析不同暴露实验中产生的复杂数据集。代谢物峰轮廓面积变化的方差分析同时成分分析表明，毒死蜱浓度和暴露时间存在显著依赖性变化，能清晰区分暴露组和未暴露组样本，以及短暴露时间（2小时）和长暴露时间（6小时或24小时）的样本。在50种肌肉代谢物浓度中观察到的变化表明存在氧化应激诱导、神经递质代谢的普遍紊乱以及肌肉疲劳。这三种效应与毒死蜱的毒性密切相关。
