Zhang Zecai, Liu Jiuxi, Shen Peng, Cao Yongguo, Lu Xiaojie, Gao Xuejiao, Fu Yunhe, Liu Bo, Zhang Naisheng
College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
Int Immunopharmacol. 2016 Dec;41:127-135. doi: 10.1016/j.intimp.2016.10.021. Epub 2016 Nov 11.
Zanthoxylum bungeanum, which belongs to the Zanthoxylum genus of the Rutaceae family, is now wildly distributed in most parts of China and some Southeast Asian countries. The pericarp of Zanthoxylum bungeanum has been known to exhibit antibacterial, anti-inflammatory and other important therapeutic activities. The purpose of this study was to investigate the effects and mechanisms of Zanthoxylum bungeanum pericarp extract (ZBE) on DSS-induced experimental colitis in mice. The results demonstrated that the major flavonoid composition of ZBE includes rutin (32.36%), quercetin (13.61%) and isoquercitrin (24.89%). ZBE alleviated DSS-induced body weight loss, colon length shortening and colonic pathological damage. Furthermore, ZBE inhibited the expression of TNF-α, IL-1β and IL-12 via the regulation of TLR4 and TLR4-related pathways in DSS-induced experimental colitis in mice and LPS-triggered inflammation in J774.1 cells. Our findings suggest that ZBE is effective in ameliorating experimental colitis, and further investigation is necessary on the use of ZBE as a new dietary strategy to lower the risk of ulcerative colitis (UC).
花椒属于芸香科花椒属植物，目前在中国大部分地区和一些东南亚国家广泛分布。已知花椒果皮具有抗菌、抗炎等重要治疗活性。本研究旨在探讨花椒果皮提取物（ZBE）对葡聚糖硫酸钠（DSS）诱导的小鼠实验性结肠炎的影响及机制。结果表明，ZBE的主要黄酮类成分包括芦丁（32.36%）、槲皮素（13.61%）和异槲皮苷（24.89%）。ZBE减轻了DSS诱导的体重减轻、结肠长度缩短和结肠病理损伤。此外，ZBE通过调节DSS诱导的小鼠实验性结肠炎中TLR4和TLR4相关途径以及J774.1细胞中脂多糖（LPS）引发的炎症，抑制了肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-12（IL-12）的表达。我们的研究结果表明，ZBE在改善实验性结肠炎方面有效，有必要进一步研究将ZBE作为一种新的饮食策略来降低溃疡性结肠炎（UC）风险的用途。
