卫生条件差对热带地区儿童细胞因子表型的影响。
Effects of poor hygiene on cytokine phenotypes in children in the tropics.
作者信息
Figueiredo C A, Amorim L D, Vaca M, Chico M E, Campos A C, Barreto M L, Cooper P J
机构信息
Instituto de Ciencias da Saude, Universidade Federal da Bahia, Salvador, Brazil.
Instituto de Matematica, Universidade Federal da Bahia, Salvador, Brazil.
出版信息
World Allergy Organ J. 2016 Nov 3;9(1):34. doi: 10.1186/s40413-016-0124-1. eCollection 2016.
We describe immune phenotypes (innate and adaptive cytokines) according to environmental exposure using latent class analysis. A total of 310 schoolchildren living in Ecuador were assayed for spontaneous cytokine production as well as mitogen (SEB)-stimulated cytokines in whole blood cultures. We collected data on environmental exposures by questionnaire and on intestinal parasites by examination of stool samples. Latent class analysis (LCA) was used to group children according to their innate (IL-6, IL-8, IL-10 and TNF-α) and adaptive (IL-5, IL-13, IL-17, IFN-γ and IL-10) cytokine profile. We also conducted multiple-group LCA and LCA with covariates to evaluate the effect of predictors on profile membership. We identified both hyporesponsive and Th2-modified immune phenotypes produced by peripheral blood leukocytes (PBLs) that were associated with intestinal worms and birth order, providing insights into how poor hygiene mediates immunologic effects on immune-mediated diseases.
我们使用潜在类别分析，根据环境暴露情况描述免疫表型（先天性和适应性细胞因子）。对生活在厄瓜多尔的310名学童进行了检测，以测定全血培养物中自发产生的细胞因子以及丝裂原（SEB）刺激产生的细胞因子。我们通过问卷调查收集了环境暴露数据，并通过检查粪便样本收集了肠道寄生虫数据。潜在类别分析（LCA）用于根据儿童的先天性（IL-6、IL-8、IL-10和TNF-α）和适应性（IL-5、IL-13、IL-17、IFN-γ和IL-10）细胞因子谱进行分组。我们还进行了多组LCA和带协变量的LCA，以评估预测因素对类别归属的影响。我们确定了外周血白细胞（PBL）产生的低反应性和Th2修饰免疫表型，这些表型与肠道蠕虫和出生顺序有关，这为不良卫生习惯如何介导对免疫介导疾病的免疫影响提供了见解。
Zotero 插件