Department of Clinical Laboratory Sciences, Faculty of Pharmacy, University of Kufa, Kufa, Iraq.
J Med Life. 2022 Apr;15(4):479-482. doi: 10.25122/jml-2021-0383.
Cytokine response to () infection was measured after starting treatments with piperazine. This study aims to determine the impact of cytokine production after infection with before and after treatment with piperazine. Blood and stool samples of 50 patients with infection and 28 healthy individuals (control) were collected. In this study, IFNγ, IL-5, IL-12, and IL-13 in serum (using ELISA-based methods) were measured. Stool samples were examined using the Kato-Katz technique to detect parasites. Blood and stool samples were analyzed 14 days after starting piperazine treatment for infection. The medium concentration of IFNγ, IL-5, IL-12, and IL-13 in the serum samples with infection is higher than that of the control group. IFNγ, IL-5, IL-12, and IL-13 levels were significantly higher in the infected individuals (10.5±7.4 pg/ml, 14.6±5.1 pg/ml, 8.5±3.2 pg/ml and 13.6±7.5 pg/ml respectively) than the control group (4.7±2.4 pg/ml, 7.8±4.06 pg/ml, 6.3±3.4 pg/ml and 3.5±2.7 pg/ml respectively). Also, piperazine treatment can significantly reduce cytokines levels (IFN-γ: P=0.043, IL-5: P=0.02, and IL-12, p=0.001). This study shows that piperazine treatment can reduce cytokines profiles in patients with infection.
本研究旨在探讨吡喹酮治疗前后感染对细胞因子产生的影响。收集了 50 例感染患者和 28 例健康个体（对照组）的血液和粪便样本。采用 ELISA 法检测血清中 IFNγ、IL-5、IL-12 和 IL-13。采用加藤厚涂片法检测粪便样本中的寄生虫。在感染患者开始服用吡喹酮治疗 14 天后，分析其血液和粪便样本。感染组血清中 IFNγ、IL-5、IL-12 和 IL-13 的中浓度高于对照组。感染组 IFNγ、IL-5、IL-12 和 IL-13 的水平明显高于对照组（分别为 10.5±7.4pg/ml、14.6±5.1pg/ml、8.5±3.2pg/ml 和 13.6±7.5pg/ml）。此外，吡喹酮治疗可显著降低细胞因子水平（IFN-γ：P=0.043，IL-5：P=0.02，IL-12，p=0.001）。本研究表明，吡喹酮治疗可降低感染患者的细胞因子谱。
