Zhou Wenli, Zhang Jiantao, Wang Guangming, Ling Limian, Yan Chaoying
Department of Neonatology, The First Hospital of Jilin University, 71 Xinmin Street, Changchun, 130021 Jilin China.
Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun, 130021 Jilin China.
Springerplus. 2016 Oct 28;5(1):1893. doi: 10.1186/s40064-016-3594-2. eCollection 2016.
To investigate the permeability of β-NGF through blood-brain-barrier (BBB) in neonatal and adult rats, and the spatial distribution of β-NGF in different brain regions in hypoxic-ischemic (HI) and normal neonatal rats.
To investigate the overall permeability of β-NGF through BBB, β-NGF labeled with I was injected into adult rats, neonatal rats and HI neonatal rats via tail vein. The radioactivity of brain tissue and blood was examined and analyzed 30 min after injection. Also, brain regions including the basal forebrain, frontal cortex, hippocampus, hypothalamus, cerebellum, bulbus olfactorius and hypophysis, of all the rats were dissected and radioactivity was examined to investigate the spatial specificity of NGF permeation through BBB.
Statistically significant results were observed in I-β-NGF contents in brain tissues of adult rats group, neonatal rats group and HI neonatal rats group (P < 0.05). Compared to the HI neonatal rats' brain with the highest I-β-NGF contents, normal neonatal rats ranks the second while the adult rats were the lowest. While for the spatial specificity examination part, I-β-NGF in both HI group and control group were widely distributed in basal forebrain, frontal cortex, hippocampus, cerebellum and bulbus olfactorius. But the radioactivity in frontal cortex, hippocampus and cerebellum of HI groups are statistically higher than control groups (P < 0.05).
β-NGF can more easily penetrate the BBB of newborn rats than adult rats via peripheral venous administration and this effect can be enhanced by HI insult. Also, this HI-induced permeation of β-NGF through BBB is more obvious in frontal cortex, hippocampus and cerebellum.
研究新生大鼠和成年大鼠中β-神经生长因子（β-NGF）通过血脑屏障（BBB）的通透性，以及缺氧缺血（HI）新生大鼠和正常新生大鼠不同脑区中β-NGF的空间分布。
为研究β-NGF通过BBB的整体通透性，将碘标记的β-NGF经尾静脉注射入成年大鼠、新生大鼠和HI新生大鼠体内。注射30分钟后检测并分析脑组织和血液的放射性。此外，解剖所有大鼠的脑区，包括基底前脑、额叶皮质、海马体、下丘脑、小脑、嗅球和垂体，并检测放射性，以研究NGF通过BBB渗透的空间特异性。
在成年大鼠组、新生大鼠组和HI新生大鼠组的脑组织中，碘-β-NGF含量观察到具有统计学意义的结果（P<0.05）。与碘-β-NGF含量最高的HI新生大鼠脑相比，正常新生大鼠位居第二，成年大鼠最低。而在空间特异性检测部分，HI组和对照组中的碘-β-NGF均广泛分布于基底前脑、额叶皮质、海马体、小脑和嗅球。但HI组额叶皮质、海马体和小脑的放射性在统计学上高于对照组（P<0.05）。
通过外周静脉给药，β-NGF比成年大鼠更容易穿透新生大鼠的BBB，且HI损伤可增强这种作用。此外，HI诱导的β-NGF通过BBB的渗透在额叶皮质、海马体和小脑中更为明显。
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