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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Wang Y C, Li Z J, Han R L, Xu C L, Wang S H, Sun G R, Wang S H, Wu J P, Kang X T

b Department of Animal Science , College of Animal Science and Technology, Gansu Agricultural University , Lanzhou , P. R. China.

a Department of Animal Science , College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou , P. R. China.

Br Poult Sci. 2017 Feb;58(1):26-31. doi: 10.1080/00071668.2016.1236363. Epub 2016 Dec 16.

This study was conducted to explore the promoter region of the chicken ASB15 gene by detecting the activities of the dual luciferase reporter gene and to assess expression profiles of the ASB15 gene in 10 different tissues from Gushi chickens. 2. Five dual luciferase reporter gene vectors were constructed and transfected into DF1 cells. The activities of recombined plasmids were measured and the core promoter was confirmed by bioinformatic analysis. Total RNA was extracted and the relative expression of the ASB15 gene was examined. 3. Data analysis indicated that the promoter was located from -955 to -212 bp. Results showed that the chicken ASB15 gene was expressed in heart, breast muscle and leg muscle. 4. This study has confirmed the promoter region and the expression profile of the chicken ASB15 gene, which provides a foundation for further exploring its transcriptional regulation and function.

本研究旨在通过检测双荧光素酶报告基因的活性来探索鸡ASB15基因的启动子区域，并评估该基因在固始鸡10种不同组织中的表达谱。2. 构建了5个双荧光素酶报告基因载体并转染至DF1细胞。测定重组质粒的活性，并通过生物信息学分析确定核心启动子。提取总RNA并检测ASB15基因的相对表达。3. 数据分析表明启动子位于-955至-212bp。结果显示鸡ASB15基因在心脏、胸肌和腿肌中表达。4. 本研究证实了鸡ASB15基因的启动子区域和表达谱，为进一步探索其转录调控和功能奠定了基础。

Wang Y C, Li Z J, Han R L, Xu C L, Wang S H, Sun G R, Wang S H, Wu J P, Kang X T

b Department of Animal Science , College of Animal Science and Technology, Gansu Agricultural University , Lanzhou , P. R. China.

a Department of Animal Science , College of Animal Science and Veterinary Medicine, Henan Agricultural University , Zhengzhou , P. R. China.

Br Poult Sci. 2017 Feb;58(1):26-31. doi: 10.1080/00071668.2016.1236363. Epub 2016 Dec 16.

This study was conducted to explore the promoter region of the chicken ASB15 gene by detecting the activities of the dual luciferase reporter gene and to assess expression profiles of the ASB15 gene in 10 different tissues from Gushi chickens. 2. Five dual luciferase reporter gene vectors were constructed and transfected into DF1 cells. The activities of recombined plasmids were measured and the core promoter was confirmed by bioinformatic analysis. Total RNA was extracted and the relative expression of the ASB15 gene was examined. 3. Data analysis indicated that the promoter was located from -955 to -212 bp. Results showed that the chicken ASB15 gene was expressed in heart, breast muscle and leg muscle. 4. This study has confirmed the promoter region and the expression profile of the chicken ASB15 gene, which provides a foundation for further exploring its transcriptional regulation and function.

本研究旨在通过检测双荧光素酶报告基因的活性来探索鸡ASB15基因的启动子区域，并评估该基因在固始鸡10种不同组织中的表达谱。2. 构建了5个双荧光素酶报告基因载体并转染至DF1细胞。测定重组质粒的活性，并通过生物信息学分析确定核心启动子。提取总RNA并检测ASB15基因的相对表达。3. 数据分析表明启动子位于-955至-212bp。结果显示鸡ASB15基因在心脏、胸肌和腿肌中表达。4. 本研究证实了鸡ASB15基因的启动子区域和表达谱，为进一步探索其转录调控和功能奠定了基础。