Ju Jaehyun, Lee Ga-Young, Kim Yoon-Se, Chang Hee Kyung, Do Myoung-Sool, Park Kun-Young
1 Department of Food Science and Nutrition, Pusan National University , Busan, Korea.
2 Department of Food Science and Biotechnology, Cha University , Seongnam, Korea.
J Med Food. 2016 Nov;19(11):1015-1022. doi: 10.1089/jmf.2016.3798.
The aim of our experiment was to evaluate the anticancer effect of bamboo salt (BS) on C57BL/6 mice in an azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon cancer model. BS, solar salt, and purified salt were evaluated for their protective effects during AOM/DSS-induced colon carcinogenesis in C57BL/6 mice. BS, especially after baking for nine separate intervals (BS9x), suppressed colon carcinogenesis in the mice. BS9x decreased colon length shortening, weight-to-length ratios, and tumor counts. Pathological evidence from histological evaluation by hematoxylin and eosin staining also revealed suppression of tumorigenesis. BS9x lowered serum levels of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) to close to those of the Normal group. Additionally, BS9x suppressed colon mRNA expression of proinflammatory factors and significantly regulated mRNA levels of the apoptosis-related factors, Bax and Bcl-2, and the cell cycle-related genes, p21 and p53. Additionally, immunohistochemistry showed that BS promoted p21 expression in the colon. Taken together, the results indicate that BS exhibited anticancer efficacy by modulating apoptosis- and inflammation-related gene expression during colon carcinogenesis in mice, and repetition in baking cycles of BS enhanced its anticancer functionality.
我们实验的目的是在氧化偶氮甲烷（AOM）/葡聚糖硫酸钠（DSS）诱导的结肠癌模型中评估竹盐（BS）对C57BL/6小鼠的抗癌作用。评估了竹盐、日晒盐和精制盐在AOM/DSS诱导的C57BL/6小鼠结肠癌发生过程中的保护作用。竹盐，尤其是经过九个不同间隔烘烤后（BS9x），抑制了小鼠的结肠癌发生。BS9x减少了结肠长度缩短、重量与长度比以及肿瘤数量。苏木精和伊红染色组织学评估的病理证据也显示肿瘤发生受到抑制。BS9x将促炎细胞因子（TNF-α、IL-6和IL-1β）的血清水平降低至接近正常组。此外，BS9x抑制结肠促炎因子的mRNA表达，并显著调节凋亡相关因子Bax和Bcl-以及细胞周期相关基因p21和p53的mRNA水平。此外，免疫组织化学显示竹盐促进结肠中p21的表达。综上所述，结果表明竹盐在小鼠结肠癌发生过程中通过调节凋亡和炎症相关基因表达表现出抗癌功效，并且竹盐烘烤周期的重复增强了其抗癌功能。
