Borque L, Cambiaso C, Lavenne E, Leautaud P, Mareschal J C, Collet-Cassart D
Unit of Experimental Medicine, International Institute for Cellular and Molecular Pathology, Bruxelles, Belgium.
J Clin Chem Clin Biochem. 1989 Mar;27(3):175-9. doi: 10.1515/cclm.1989.27.3.175.
Based on immunoassay by particle counting, three methods for antithrombin III, von Willebrand factor and plasminogen were developed on an automated IMPACT machine and on a semi-automated MULTIPACT system. Precision of the techniques, measured at low, medium and high level of the calibration curve showed coefficients of variation varying from 4.3 to 13.8%. Accuracy was evaluated by dilution recovery test and by correlation with rocket immunoelectrophoresis and chromogenic substrate techniques. The results show that the proposed methods correlate well with existing techniques and that immunoassay by particle counting is applicable to several coagulation tests.
基于颗粒计数免疫测定法，在自动IMPACT仪器和半自动MULTIPACT系统上开发了抗凝血酶III、血管性血友病因子和纤溶酶原的三种检测方法。在校准曲线的低、中、高水平下测量这些技术的精密度，变异系数在4.3%至13.8%之间。通过稀释回收率测试以及与火箭免疫电泳和发色底物技术的相关性来评估准确性。结果表明，所提出的方法与现有技术相关性良好，且颗粒计数免疫测定法适用于多种凝血检测。
