Stahlman M T, Orth D N, Gray M E
Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee.
Lab Invest. 1989 Apr;60(4):539-47.
Cells staining for immunoreactive human epidermal growth factor were sought in the lungs and tracheas of human fetuses from 8 to 24 weeks of gestation. Lungs of liveborn infants from 25 to 40 weeks of gestation (stillborn to 7 months postnatal life), both with and without lung pathology, were also studied. In the early fetal trachea (12 to 15 weeks), many nonciliated cells immunostained for immunoreactive human epidermal growth factor in the lining epithelium. By 16 weeks of gestation this widespread staining was replaced by stained nonciliated single cells or small clusters of cells which were identifiable until 24 weeks. In the few tracheas which were available from liveborn infants who died without evidence of lung disease, stained cells were seldom identified in the lining epithelium after 24 weeks of gestation. In contrast, from 18 weeks until term, tracheal submucosal glands contained scattered cells which immunostained for immunoreactive human epidermal growth factor but which did not appear to be classical mucous cells. Beginning at 20 weeks of gestation, positively staining cells were found occasionally in bronchial lining epithelium, but more often in bronchial submucosal glands. Immunostained cells were never identified in bronchiolar epithelium in normal fetal or newborn lungs. In liveborn infants from 24 weeks onward who developed lung disease, many tracheas were severely damaged. In the presence of extensive denudation of the mucosa or the development of squamous metaplasia, immunostained cells were rarely seen in the lining epithelium. However, even under these conditions stained glandular cells could usually be identified. Stained cells were also present in the necks of those tracheal glands from which new epithelial lining cells appeared to be migrating onto denuded surfaces. Immunostained cells in the bronchial lining epithelium of infants with chronic lung disease were infrequent, just as they were in the fetus, but bronchial submucosal glands contained positively stained cells similar to those in tracheal glands. The appearance and distribution of immunostained cells were similar in the tracheal and bronchial submucosal glands in both normal subjects and those with all stages of lung disease. In contrast to the bronchioles of fetuses and infants without lung pathology, the bronchiolar epithelium of infants with chronic lung disease contained immunostained cells. Immunostained cells were found in areas of migrating dysplastic cells in relining conducting airways.(ABSTRACT TRUNCATED AT 400 WORDS)
研究人员在妊娠8至24周的人类胎儿的肺和气管中寻找免疫反应性人表皮生长因子染色的细胞。还对妊娠25至40周（死产至出生后7个月）的活产婴儿的肺进行了研究，这些婴儿既有肺部病变的，也有无肺部病变的。在胎儿早期气管（12至15周），内衬上皮中的许多非纤毛细胞免疫染色显示有免疫反应性人表皮生长因子。到妊娠16周时，这种广泛的染色被染色的非纤毛单个细胞或小细胞簇所取代，这些细胞一直可被识别到24周。在死于无肺部疾病证据的活产婴儿的少数气管中，妊娠24周后在内衬上皮中很少能识别到染色细胞。相比之下，从18周直到足月，气管黏膜下腺含有散在的细胞，这些细胞免疫染色显示有免疫反应性人表皮生长因子，但似乎不是典型的黏液细胞。从妊娠20周开始，在支气管内衬上皮中偶尔发现阳性染色细胞，但在支气管黏膜下腺中更常见。在正常胎儿或新生儿的肺中，细支气管上皮中从未发现免疫染色细胞。在24周及以后患肺部疾病的活产婴儿中，许多气管严重受损。在存在广泛黏膜剥脱或鳞状化生的情况下，内衬上皮中很少见到免疫染色细胞。然而，即使在这些情况下，通常也能识别出染色的腺细胞。在那些气管腺的颈部也有染色细胞，新的上皮衬里细胞似乎正从这些部位迁移到剥脱表面。患有慢性肺部疾病的婴儿的支气管内衬上皮中的免疫染色细胞很少见，就像在胎儿中一样，但支气管黏膜下腺含有与气管腺中相似的阳性染色细胞。在正常受试者和患有各阶段肺部疾病的受试者中，气管和支气管黏膜下腺中免疫染色细胞的外观和分布相似。与无肺部病变的胎儿和婴儿的细支气管不同，患有慢性肺部疾病的婴儿的细支气管上皮含有免疫染色细胞。在重新衬里的传导气道中，在发育异常细胞迁移的区域发现了免疫染色细胞。（摘要截选至400字）
