van Buul-Wortelboer M F, Brinkman H J, Reinders J H, van Aken W G, van Mourik J A
Department of Blood Coagulation, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Biochim Biophys Acta. 1989 May 10;1011(2-3):129-33. doi: 10.1016/0167-4889(89)90199-7.
Human umbilical vein endothelial cells cultured on a collagen lattice were used to study the polarity of von Willebrand factor (vWF) secretion. Endothelial cells cultured under these conditions allow direct measurements of substances released at both the apical and basolateral surface. The constitutive secretion of vWF was compared to the release of vWF from their storage granules after stimulation (regulated secretion). The basal, constitutive release of vWF occurs into both the apical and subendothelial direction. The rate of accumulation of vWF to the subendothelial direction is about three times higher than the amount of vWF secreted into the lumenal medium per unit of time. However, upon stimulation of confluent endothelial cell monolayers with phorbol myristate acetate, endothelial cells predominantly secrete vWF at the lumenal surface. Under these conditions, vWF does not accumulate in the collagen matrix. Thus, endothelial cells are able to organize themselves into a polarized monolayer, in such a way that vWF secreted by the regulated pathway accumulates at the lumenal site, whereas resting endothelial cells release vWF predominantly at the opposite, basolateral surface.
在胶原晶格上培养的人脐静脉内皮细胞被用于研究血管性血友病因子（vWF）分泌的极性。在这些条件下培养的内皮细胞允许直接测量在顶端和基底外侧表面释放的物质。将vWF的组成性分泌与刺激后其储存颗粒中vWF的释放（调节性分泌）进行比较。vWF的基础组成性释放发生在顶端和内皮下两个方向。vWF向内皮下方向积累的速率比每单位时间分泌到管腔介质中的vWF量高约三倍。然而，用佛波酯肉豆蔻酸酯刺激汇合的内皮细胞单层时，内皮细胞主要在管腔表面分泌vWF。在这些条件下，vWF不会在胶原基质中积累。因此，内皮细胞能够组织成极化的单层，使得通过调节途径分泌的vWF在管腔部位积累，而静息的内皮细胞主要在相对的基底外侧表面释放vWF。
