Matsumoto Y, Kawai K, Fujiwara M
Department of Immunology, Niigata University School of Medicine, Japan.
Immunology. 1989 Apr;66(4):621-7.
In order to examine in situ Ia expression on brain cells of various strains of rat, experimental autoimmune encephalomyelitis (EAE) was induced in both EAE-susceptible (LEW) and EAE-resistant (BN) strains. For induction of EAE in the resistant strain, two methods were applied: one was injection of guinea-pig myelin basic protein (GPBP) in complete Freund's adjuvant into LBNF1----BN chimeras; the other was transfer of GPBP-reactive T-line cells from BN rats into syngeneic rats. LBNF----BN chimeras developed clinical EAE, whereas BN rats that received T-line cells did not. However, histological EAE was apparent in both groups. Immunohistochemical examination using two different monoclonal antibodies (OX3 and OX6) against rat Ia antigens revealed that microglia of LEW, BN and chimera rats expressed Ia antigens in the central nervous system (CNS) with EAE. On the other hand, astrocytes were negative for Ia antigens in all the strains. Furthermore, quantitative analysis was undertaken in order to compare the density of Ia-positive microglia in the BN CNS with that in the LEW CNS. It was revealed that the density of Ia-positive microglia in the vicinity of perivascular inflammatory cell aggregates was essentially the same in both strains regardless of the difference in methods of EAE induction or histological severity of the disease. Ia-positive microglia remote from inflammatory cell aggregates were somewhat fewer in rats with mild histological EAE. However, no strain difference was noted in this analysis. Therefore, we concluded that in situ Ia-inducibility on the brain cells of EAE-resistant rats is not different from that of EAE-susceptible rats. Although Ia-positive microglia in both strains may be involved in the immune responses in the CNS, it is unlikely that the difference in Ia-inducibility on brain cells would contribute to strain-specific susceptibility to EAE.
为了检测不同品系大鼠脑细胞原位Ia表达情况，在易患实验性自身免疫性脑脊髓炎（EAE）的品系（LEW）和抗EAE的品系（BN）中均诱导了EAE。为了在抗性品系中诱导EAE，应用了两种方法：一种是将豚鼠髓鞘碱性蛋白（GPBP）与完全弗氏佐剂一起注射到LBNF1-BN嵌合体中；另一种是将来自BN大鼠的GPBP反应性T系细胞转移到同基因大鼠中。LBNF-BN嵌合体出现了临床EAE，而接受T系细胞的BN大鼠则未出现。然而，两组均出现了组织学EAE。使用两种针对大鼠Ia抗原的不同单克隆抗体（OX3和OX6）进行免疫组织化学检查发现，LEW、BN和嵌合体大鼠的小胶质细胞在患有EAE时在中枢神经系统（CNS）中表达Ia抗原。另一方面，所有品系的星形胶质细胞Ia抗原均为阴性。此外，进行了定量分析以比较BN中枢神经系统中Ia阳性小胶质细胞的密度与LEW中枢神经系统中的密度。结果显示，无论EAE诱导方法的差异或疾病的组织学严重程度如何，两个品系中血管周围炎性细胞聚集附近的Ia阳性小胶质细胞密度基本相同。在组织学EAE较轻的大鼠中，远离炎性细胞聚集的Ia阳性小胶质细胞略少。然而，该分析未发现品系差异。因此，我们得出结论，抗EAE大鼠脑细胞的原位Ia诱导能力与易患EAE大鼠的无异。尽管两个品系中的Ia阳性小胶质细胞可能都参与了中枢神经系统的免疫反应，但脑细胞Ia诱导能力的差异不太可能导致品系特异性的EAE易感性。
